Effect of estrogen on prolactin mRNA in the rat pituitary. Analysis by in situ hybridization and immunohistochemistry.
Effect of estrogen on prolactin mRNA in the rat pituitary. Analysis by in situ hybridization and immunohistochemistry.
复制标题
雌激素对大鼠垂体催乳素 mRNA 的影响。
DOI：
10.1159/000124493
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发表时间：
1986
影响因子：
4.1
通讯作者：
N. Kageyama
中科院分区：
文献类型：
--
作者：
N. Yamamoto;H. Seo;N. Suganuma;N. Matsui;T. Nakane;A. Kuwayama;N. Kageyama
采用原位杂交和免疫组织化学方法，从单细胞水平研究雌激素对催乳素(PRL)合成的影响。免疫组织化学显示，长期雌激素治疗使含有PRL的细胞从细胞总数的10%-20%增加到80%-90%。含有PRL mRNA的细胞也以类似的方式增加。此外，在雌激素作用下，胞浆内PRL基因的表达增加了4-5倍，表现为细胞内银粒数的增加。这些结果表明，长期雌激素治疗不仅会导致PRL细胞的增殖，而且会导致单个细胞中PRL mRNA的增加。
The effect of estrogen on prolactin (PRL) synthesis at a single-cell level was studied by in situ hybridization and immunohistochemistry. Long-term estrogen treatment increased PRL-containing cells from 10-20% of total cell population to 80-90%, as revealed by immunohistochemistry. PRL mRNA containing cells also increased in a similar fashion. Moreover, cytoplasmic PRL mRNA expressed as the number of silver grains per cell increased 4- to 5-fold by estrogen. These results suggest that long-term estrogen treatment causes not only PRL cell proliferation but also an increase in PRL mRNA in a single cell.