Construction of an Escherichia coli mutant producing monophosphoryl lipid A
Construction of an Escherichia coli mutant producing monophosphoryl lipid A
复制标题
产生单磷酰脂质 A 的大肠杆菌突变体的构建。
DOI：
10.1007/s10529-011-0521-z
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发表时间：
2011-05-01
影响因子：
2.7
通讯作者：
Wang, Xiaoyuan
中科院分区：
文献类型：
--
作者：
Chen, Jiuzhou;Tao, Guanjun;Wang, Xiaoyuan
类脂A是大多数革兰氏阴性细菌外膜的主要成分。单磷脂A在1-位不含磷酸基团，可用作佐剂。我们通过将lpxE基因整合到大肠杆菌W3110的染色体上，构建了一株突变体CW001。LpxE基因编码一种酶LpxE，它可以去除脂质A的1-磷酸基团。薄层色谱和电喷雾电离质谱仪表明，CW001在体内主要产生1-去磷酸化的脂质A。本研究不仅对脂类A佐剂的开发具有重要意义，而且为将外源基因整合到大肠杆菌染色体中提供了一种新的方法。
Lipid A is a major component in the outer membrane of most Gram-negative bacteria. Monophosphoryl lipid A contains no phosphate group at 1-position and can be used as an adjuvant. We constructed an Escherichia coli mutant CW001 by integrating a gene lpxE into the chromosome of E. coli W3110. The gene lpxE encodes an enzyme LpxE which removes the 1-phosphate group of lipid A. CW001 predominantly produces 1-dephosphorylated lipid A in vivo, as adjudged by thin layer chromatography and electro-spray ionization mass spectrometry. This study not only is important for the development of lipid A adjuvants but also provides a novel method for integration of heterologous genes into the chromosome of E. coli.