Genomic analysis of detoxification genes in the mosquito Aedes aegypti
Genomic analysis of detoxification genes in the mosquito Aedes aegypti
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DOI：
10.1016/j.ibmb.2007.09.007
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发表时间：
2008-01-01
影响因子：
3.8
通讯作者：
Ranson, Hilary
中科院分区：
文献类型：
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作者：
Strode, Clare;Wondji, Charles S.;Ranson, Hilary
最近确定的主要登革热载体，埃及伊蚊的基因组序列的注释，揭示了大量的解毒基因。在这里，我们报告的存在下，235个成员的细胞色素P450，谷胱甘肽转移酶和羧基/胆碱酯酶家族在AE。埃及人。与果蝇（Drosophila melanogaster）和疟蚊（Anopheles gambiae）相比，该基因数量分别增加了58%和36%。在基因家族中，这种扩增是不均匀的。可以在昆虫物种中发现具有假定或证实的生物合成或管家作用的酶的安全直系同源物。与此相反，这些基因家族的子集，与一般异生物质解毒，特别是CYP 6，CYP 9和α酯酶家族，扩大了在Ae。埃及人。为了鉴定与杀虫剂抗性相关的解毒基因，我们构建了含有这些基因的独特寡核苷酸探针的阵列，并比较了它们在杀虫剂抗性和敏感菌株中的表达水平。确定了几个候选基因，其中大多数属于两个基因家族，CYP 9 P450和Epidermal GST。这是阿。“埃及排毒芯片”将促进虫媒病毒控制方案中杀虫剂抗药性管理战略的实施。(c)2007爱思唯尔有限公司保留所有权利。
Annotation of the recently determined genome sequence of the major dengue vector, Aedes aegypti, reveals an abundance of detoxification genes. Here, we report the presence of 235 members of the cytochrome P450, glutathione transferase and carboxy/ cholinesterase families in Ae. aegypti. This gene count represents an increase of 58% and 36% compared with the fruitfly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae, respectively. The expansion is not uniform within the gene families. Secure orthologs can be found across the insect species for enzymes that have presumed or proven biosynthetic or housekeeping roles. In contrast, subsets of these gene families that are associated with general xenobiotic detoxification, in particular the CYP6, CYP9 and alpha esterase families, have expanded in Ae. aegypti. In order to identify detoxification genes associated with resistance to insecticides we constructed an array containing unique oligonucleotide p robes for these genes and compared their expression level in insecticide resistant and susceptible strains. Several candidate genes were identified with the majority belonging to two gene families, the CYP9 P450s and the Epsilon GSTs. This 'Ae. aegypti Detox Chip' will facilitate the implementation of insecticide resistance management strategies for arboviral control programmes. (c) 2007 Elsevier Ltd. All rights reserved.