STABLE VARIANTS AFFECTING B-CELL ALLOANTIGENS IN HUMAN LYMPHOID-CELLS
STABLE VARIANTS AFFECTING B-CELL ALLOANTIGENS IN HUMAN LYMPHOID-CELLS
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DOI：
10.1038/271459a0
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发表时间：
1978-01-01
期刊：
影响因子：
64.8
通讯作者：
PIOUS, D
中科院分区：
文献类型：
--
作者：
GLADSTONE, P;PIOUS, D
人类主要组织相容性复合体 (MHC) 由四个位点定义：HLA-A、BandCare 血清学定义以及 HLA-D 最初由混合淋巴细胞反应 (MLR)1 定义。现在有证据表明一组HLA连锁的、血清学上确定的B细胞特异性同种抗原是D基因座本身或紧密连锁的基因的基因产物2,3。在 HLA-D 等位基因和 B 细胞特异性基因型之间观察到很强的关联，并且 B 细胞抗血清抑制 MLR4,5。 D相关抗原与HLA-A、BandC的产物在化学结构和组织分布上有所不同。人类 B 细胞同种异体抗原类似于小鼠的 Ia 抗原。由于重组个体的可用性，在人和小鼠中对 Ia 抗原、MLC 位点和 I 区其他功能定位之间关系的分析受到限制。由于此类重组体很少见，我们已开始通过突变分析该区域，使用免疫选择来分离人淋巴细胞系中 MHC 基因产物缺陷的突变体。我们在这里描述了在任何物种中分离和表征的第一个 Ia 变体。
THE human major histocompatibility complex (MHC) is defined by four loci:HLA-A,BandCare serologically defined andHLA-Dwas initially defined by the mixed lymphocyte reaction (MLR)1. There is now evidence that a group of HLA-linked, serologically defined B cell-specific alloantigens are the gene products of either theDlocus itself or of closely linked genes2,3. Strong associations are seen between alleles ofHLA-Dand the B cell-specific genotypes, and B cell antisera inhibit the MLR4,5. TheD-associated antigens differ from the products ofHLA-A,BandCin chemical structure and in tissue distribution6. Human B-cell alloantigens resemble Ia antigens in mice. Analysis of the relationships among Ia antigens, MLC loci and other functions mapping in the I region is limited in both humans and mice by the availability of recombinant individuals7. Because such recombinants are rare, we have begun an analysis of this region by mutation8, using immuno-selection to isolate mutants defective for MHC gene products in human lymphoid cell lines. We describe here the first Ia variants to be isolated and characterised in any species.