TMT29 plex: Higher order multiplexing with inherent interference correction.
TMT29 plex: Higher order multiplexing with inherent interference correction.
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TMT29 plex：具有固有干扰校正功能的高阶复用。
DOI：
10.1002/pmic.202200260
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发表时间：
2022
期刊：
影响因子：
3.4
通讯作者：
Zhang,Tian
中科院分区：
文献类型：
--
作者：
Zhang,Tian
串联质量标签(TMT)技术使基于质谱学的多路样品分析蛋白质丰度成为可能。TMT等压标记的程度已经从2个增加到6个、11个、16个[1]和最近的18个[2]通道，使得更高吞吐量分析的发展成为可能。在TMT实验中，报告标签强度的测量受到比率压缩的干扰。虽然已经开发了采集前和采集后的方法来减少干扰，但比率压缩在复杂样本中仍然是一个问题。Sun等人的工作。提出了一种TMT29-plex工作流程，它结合了TMT11-plex和TMTpro18-plex的标记策略，并利用它们的固有特性来解决比率压缩问题，增加了TMT的吞吐量和潜在应用的定量准确性[3]。
Tandem mass tag (TMT) technology enables mass spectrometry‐based multiplexed sample profiling of protein abundance. The degree of isobaric labeling with TMT has increased from 2 to 6, 11, 16 [1] and recently 18 [2] channels, enabling the development of higher throughput assays. In TMT experiments, measurement of reporter tag intensities is confounded by ratio compression. Although both pre‐ and post‐acquisition methods have been developed to decrease interference, ratio compression remains a problem in complex samples. The work of Sun et al. presented a TMT29‐plex workflow that combines the TMT11‐plex and TMTpro18‐plex labeling strategies and uses their inherent features to address the ratio compression problem, increasing the TMT throughput and quantitative accuracy for potential applications [3].