MORPHOLOGICAL CHANGES IN CULTURED MAMMALIAN-CELLS - PREVENTION BY CALCIUM IONOPHORE A23187
MORPHOLOGICAL CHANGES IN CULTURED MAMMALIAN-CELLS - PREVENTION BY CALCIUM IONOPHORE A23187
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DOI：
10.1016/0092-8674(75)90085-9
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发表时间：
1975-01-01
期刊：
影响因子：
64.5
通讯作者：
FREESE, E
中科院分区：
文献类型：
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作者：
HENNEBERRY, RC;FISHMAN, PH;FREESE, E
微摩尔浓度的二价阳离子载体A23187可阻止丁酸盐在HeLa细胞中和单丁酰或双丁酰cAMP在CHO细胞中引起的形态学变化。离子载体不能阻止缺少钙的培养基中的这种变化。在稍高的浓度（但无毒），离子载体inhlblts丁酸介导的诱导神经节苷脂生物合成酶，唾液酸转移酶，在HeLa。在CHO中，唾液酸转移酶活性通常很高，并且不受任何测试化合物的影响。
The morphological changes induced by butyrate In HeLa cells and by monobutyryl or dibutyryl CAMP in CHO cells are prevented by mlcromolar concentrations of the dlvalent cation ionophore A23187. The lonophore is unable to prevent such changes in medium from which calcium Is omitted. At sllghtly higher (but nontoxic) concentrations, the ionophore lnhlblts the butyrate-mediated induction of the ganglloside biosynthetic enzyme, sialyltransferase, in HeLa. In CHO, sialyltransferase activity is normally high and not altered by any of the compounds tested.