ALTERED CELL-CYCLE PROGRESSION AND ABERRANT MITOSIS IN ADENOVIRUS-INFECTED RODENT CELLS
ALTERED CELL-CYCLE PROGRESSION AND ABERRANT MITOSIS IN ADENOVIRUS-INFECTED RODENT CELLS
复制标题
DOI：
10.1002/jcp.1041110114
复制
发表时间：
1982-01-01
影响因子：
5.6
通讯作者：
TAYLOR, IW
中科院分区：
文献类型：
--
作者：
MURRAY, JD;BELLETT, AJD;TAYLOR, IW
活跃生长的小鼠或大鼠胚胎细胞在感染人5型腺病毒后遭受染色体结构损伤、有丝分裂异常和多倍性。染色体损伤需要> 1个早期病毒基因的表达，并在其频率上显示出规则的周期性。一些感染细胞的生长周期时间缩短了约5小时，由于G1的减少，连续的染色体损伤波之间的间隔对应于这个减少的周期时间。感染后，G1期细胞DNA含量下降，G2期二倍体、非整倍体和多倍体细胞DNA含量增加。这些效应似乎是由于早期病毒基因在半容许细胞中的表达，其在体内生产性感染中的功能是改变细胞周期控制，以使能够复制病毒DNA的细胞数量和这些细胞在DNA复制中花费的时间最大化。
Actively growing mouse or rat embryo cells suffered structural chromosome damage, mitotic anomalies and polyploidy after infection by human adenovirus type 5. Chromosome damage required expression of > 1 early viral genes and showed regular periodicity in its frequency. The growth cycle time of some of the infected cells was reduced by .apprx. 5 h due to a decrease in G1, and the interval between successive waves of chromosome damage corresponded to this reduced cycle time. After infection there was a decreased in cells with G1 DNA content and an increase in cells with G2 diploid, aneuploid and polyploid DNA contents. These effects appear to be due to the expression in semipermissive cells of early viral gene(s) whose function in productive infection in vivo is to alter cell cycle controls in order to maximize the number of cells able to replicate viral DNA and the time such cells spend in DNA replication.