Stromal inhibition of epithelial cell growth in the prostate; Overview of an experimental study
Stromal inhibition of epithelial cell growth in the prostate; Overview of an experimental study
复制标题
DOI：
10.1007/bf00941995
复制
发表时间：
1997-04-01
影响因子：
--
通讯作者：
Schroder, FH
中科院分区：
文献类型：
--
作者：
Kooistra, A;Romijn, JC;Schroder, FH
研究前列腺基质对前列腺上皮细胞增殖的旁分泌影响。双层软琼脂实验表明，人前列腺基质细胞抑制前列腺肿瘤上皮细胞株PC-3和LNCaP的非锚定生长。正如在多孔板上进行的半自动比色MTT试验所显示的那样，锚定依赖性生长也受到抑制。抗增殖活性是由基质细胞条件培养基中的扩散因子介导的，并且发现前列腺基质细胞特异性地产生。虽然这种被认为的抑制因子与转化生长因子（tgf - β）有一些共同的特性，但有证据表明，该因子与这种众所周知的上皮细胞生长抑制剂不同。tgf - β敏感水貂肺细胞系CCL-64对前列腺基质细胞条件培养基和浓缩部分纯化的抑制剂制剂缺乏抑制反应，表明缺乏tgf - β活性。此外，针对tgf - β 1或tgf - β 2的中和抗体不会引起部分纯化抑制剂引起的PC-3生长抑制水平的下降。结论：前列腺基质源性因子可能是一种不同于目前描述的任何抑制因子的新型生长抑制剂。
The paracrine influence of prostatic stroma on the proliferation of prostatic epithelial cells was investigated. Using a double-layer soft agar assay it was demonstrated that stromal cells from the human prostate inhibit the anchorage-independent growth of the prostatic tumor epithelial cell lines PC-3 and LNCaP. Anchorage-dependent growth was inhibited too as was shown in the semi-automated colorimetric MTT test performed on multiwell plates, Antiproliferative activity was mediated by a diffusible factor in the stromal cell conditioned medium and was found to be produced specifically by prostatic stromal cells. Although the putative inhibiting factor shared some properties with transforming growth factor beta (TGF-beta) evidence is presented that the factor is different from this well-known inhibitor of epithelial cell growth. Absence of TGF-beta activity was shown by the lack of inhibitory response of the TGF-beta-sensitive mink lung cell line CCL-64 to prostate stromal cell conditioned medium and to concentrated partially purified preparations of the inhibitor. Furthermore, neutralizing antibodies against TGF-beta 1 or TGF-beta 2 did not cause a decline in the level of PC-3 growth inhibition caused by partially purified inhibitor. It is concluded that the prostate stroma-derived factor may be a novel growth inhibitor different from any of the currently described inhibiting factors.