Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer
Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer
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DOI：
10.1007/s00428-008-0687-5
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发表时间：
2008-12-01
期刊：
影响因子：
3.5
通讯作者：
Aoki, Daisuke
中科院分区：
文献类型：
--
作者：
Fujii, Takuma;Saito, Miyuki;Aoki, Daisuke
人乳头瘤病毒DNA整合到宿主基因组中对宫颈癌的发展至关重要。P16蛋白的过表达在宫颈上皮内瘤变（CIN）和宫颈癌中均有报道。这种分子生物标志物已被用于液基细胞学标本的辅助检测;然而，它们的临床应用仍然存在争议。为检测CIN 2或更晚期病变，对153例液基细胞学（LBC）标本进行了研究，以通过原位杂交（ISH）确定人乳头瘤病毒（HPV）DNA的物理状态，并通过免疫细胞化学结合聚合酶链反应（PCR）进行HPV基因分型来检测P16蛋白的过度表达。ISH、P16免疫细胞化学和LBC联合应用具有较高的敏感性（89.3%）和特异性（92.6%）。我们证实了P16免疫细胞化学结合ISH和HPV基因分型作为辅助分子生物学检测LBC标本识别宫颈癌高危患者的有用性。
Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.