Eicosanoids in exhaled breath condensate and bronchoalveolar lavage fluid of patients with primary lung cancer
Eicosanoids in exhaled breath condensate and bronchoalveolar lavage fluid of patients with primary lung cancer
复制标题
DOI：
10.1155/2012/562862
复制
发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Antczak, Adam
中科院分区：
文献类型：
--
作者：
Ciebiada, Maciej;Gorski, Pawel;Antczak, Adam
虽然类二十烷酸与肺癌发生有关，但在原发性肺癌患者的呼出液（EBC）和支气管肺泡灌洗液（BALf）中对其研究甚少。本研究纳入17例诊断为非小细胞肺癌的患者，10例健康吸烟者和12例健康非吸烟者。采用酶联免疫吸附法测定所有患者和半数肺癌患者在任何治疗前的EBC中cys- lt、8-异前列腺素、LTB4和PGE2的水平。EBC和BALf检测到8-异前列腺素、LTB4、cys- lt和PGE2。健康吸烟者和非吸烟者在所有测量介质的浓度上没有显著差异。与健康对照组相比，诊断为肺癌的患者EBC中cys- lt （p < 0.05）和LTB4 （p < 0.05）浓度较高。肺癌患者BALf中所有介质的平均浓度均显著高于EBC， BALf中cys- lt、LTB4和8-异前列腺素浓度与EBC中cys- lt、LTB4和8-异前列腺素浓度呈显著正相关（r = 0.64, p < 0.05, r = 0.59, p < 0.05, r = 0.53, p < 0.05）。由于肺癌患者EBC中的cys-LT、LTB4和8-异前列腺素浓度反映了它们在BALf中的浓度，因此它们可能作为一种可能的非侵入性方法来监测疾病并评估治疗的有效性。
Although eicosanoids are involved in lung carcinogenesis they were poorly investigated in exhaled breath condensate (EBC) and bronchoalveolar lavage fluid (BALf) in patients with primary lung cancer. In this study 17 patients with diagnosed non-small cell lung cancer, 10 healthy smokers and 12 healthy nonsmokers were included. The levels of cys-LTs, 8-isoprostane, LTB4 and PGE2 were measured before any treatment in the EBC of all patients and in BALf of patients with lung cancer by enzyme linked immunosorbent assay. 8-isoprostane, LTB4, cys-LTs and PGE2 were detectable in the EBC and BALf. There were no significant differences between healthy smokers and nonsmokers in concentrations of all measured mediators. Compared with both healthy controls, patients with diagnosed lung cancer displayed higher concentrations of cys-LTs (p < 0.05) and LTB4 (p < 0.05) in EBC. In patients with lung cancer, the mean concentrations of all measured mediators were significantly higher in BALf compared with EBC and there was a significant, positive correlation between concentration of cys-LTs, LTB4 and 8-isoprostane in BALf and their concentrations in the EBC (r = 0.64, p < 0.05, r = 0.59, p < 0.05, r = 0.53, p < 0.05 respectively). Since cys-LT, LTB4 and 8-isoprostane concentrations in EBC from patients with lung cancer reflect their concentrations in BALf, they may serve as a possible non-invasive method to monitor the disease and to assess the effectiveness of therapy.