Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies
Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies
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DOI：
10.1007/s00216-015-8987-5
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发表时间：
2015-10-01
影响因子：
4.3
通讯作者：
Popp, Juergen
中科院分区：
文献类型：
--
作者：
Bocklitz, Thomas;Braeutigam, Katharina;Popp, Juergen
肿瘤的分子异质性是肿瘤诊断和治疗的主要障碍。为了处理这种异质性，迫切需要不同分析技术的多学科组合，因为这种组合可以创建肿瘤的多模态图像。在这里，我们开发了一个计算工作流程，以便将基质辅助激光解吸/电离质谱（MALDI-MS）成像和拉曼显微光谱成像相结合，用于组织研究。计算工作流可用于基于一种技术与另一种技术确认光谱组织病理学（SHP）。在这篇文章中，我们用maldi成像证实了基于拉曼光谱的SHP。由于这种结合，我们可以证明，对于喉癌样本，组织类型和不同的代谢状态可以从拉曼光谱中提取出来。在MALDI光谱的帮助下，进一步的研究可以更好地表征可变上皮分化，并更好地理解正在进行的发育不良改变。
Molecular heterogeneity of cancer is a major obstacle in tumor diagnosis and treatment. To deal with this heterogeneity, a multidisciplinary combination of different analysis techniques is of urgent need because a combination enables the creation of a multimodal image of a tumor. Here, we develop a computational workflow in order to combine matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) imaging and Raman microspectroscopic imaging for tissue based studies. The computational workflow can be used to confirm a spectral histopathology (SHP) based on one technique with another technique. In this contribution, we confirmed a Raman spectroscopic based SHP with MALDI-imaging. Owing to this combination, we could demonstrate, for a larynx carcinoma sample, that tissue types and different metabolic states could be extracted from the Raman spectra. Further investigations with the help of MALDI spectra yield a better characterization of variable epithelial differentiation and a better understanding of ongoing dysplastic alterations.