Pancreatic Stellate Cells Increase the Invasion of Human Pancreatic Cancer Cells through the Stromal Cell-Derived Factor-1/CXCR4 Axis
Pancreatic Stellate Cells Increase the Invasion of Human Pancreatic Cancer Cells through the Stromal Cell-Derived Factor-1/CXCR4 Axis
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DOI：
10.1159/000236012
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发表时间：
2010-01-01
期刊：
影响因子：
3.6
通讯作者：
Hu, Guoyong
中科院分区：
文献类型：
--
作者：
Gao, Zhenjun;Wang, Xingpeng;Hu, Guoyong
目的：胰腺星状细胞（PSC）和基质细胞衍生因子-1（SDF-1）/CXCR4受体配体系统在胰腺癌进展中发挥重要作用。本研究旨在检测PSC是否表达SDF-1并通过SDF-1/CXCR4受体配体轴促进胰腺癌的侵袭。方法：采用RT-PCR检测PSC、胰腺癌株和癌组织样本中SDF-1和CXCR4的表达。使用 ELISA 检测 PSC 上清液中 SDF-1 的浓度。采用MTT法检测胰腺癌细胞的增殖情况。采用Transwell小室迁移实验检测AsPC-1细胞的迁移。采用体外侵袭实验检测AsPC-1细胞的侵袭能力。结果：PSCs中检测到CXCR4表达； AsPC-1、SW1990 和 BxPC-3 癌细胞；和癌组织。在 PSC 和癌组织中检测到 SDF-1，但在 AsPC-1、SW1990 和 BxPC-3 细胞中未检测到。在 PSC 上清液中发现了 SDF-1 α 蛋白。 PSC条件培养基可以促进胰腺癌细胞的增殖、迁移和侵袭。 SDF-1中和抗体或AMD3100可以显着抑制这些促进作用。结论：PSCs能够分泌SDF-1，通过SDF-1/CXCR4轴增加胰腺癌细胞的侵袭能力。版权所有 (C) 2010 S. Karger AG，巴塞尔和 IAP
Aim: Both pancreatic stellate cells (PSCs) and the stromal cell-derived factor-1(SDF-1)/CXCR4 receptor ligand system have important roles in pancreatic cancer progression. This study set out to detect if PSCs express SDF-1 and promote the invasion of pancreatic cancer through the SDF-1/CXCR4 receptor ligand axis. Methods: RT-PCR was performed to detect the expression of SDF-1 and CXCR4 in PSCs, pancreatic cancer lines and cancer tissue samples. ELISA was used to investigate the concentration of SDF-1 in PSC supernatants. An MTT assay was applied to detect the proliferation of pancreatic cancer cells. A transwell chamber migration assay was employed to detect the migration of AsPC-1 cells. An in vitro invasion assay was used to detect the invasion of AsPC-1 cells. Results: CXCR4 expression was detected in PSCs; AsPC-1, SW1990 and BxPC-3 cancer cells; and cancer tissues. SDF-1 was detected in PSCs and cancer tissues, but not in AsPC-1, SW1990 and BxPC-3 cells. SDF-1 alpha protein was found in PSC supernatants. PSC-conditioned media can promote the proliferation, migration and invasion of pancreatic cancer cells. SDF-1 neutralizing antibody or AMD3100 can significantly inhibit these promotive effects. Conclusion: PSCs can secrete SDF-1 and increase the invasion of pancreatic cancer cells through the SDF-1/CXCR4 axis. Copyright (C) 2010 S. Karger AG, Basel and IAP