Association of the single-nucleotide polymorphism and haplotype of the P-selectin gene with ischemic stroke
Association of the single-nucleotide polymorphism and haplotype of the P-selectin gene with ischemic stroke
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DOI：
10.1007/s11239-007-0168-8
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发表时间：
2007
影响因子：
4
通讯作者：
Yesheng Wei;Y. Lan;Rui-ya Huang;Yun-guang Liu;Ren-guang Tang;Qun-qing Xu;Lan-qing Meng
中科院分区：
文献类型：
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作者：
Yesheng Wei;Y. Lan;Rui-ya Huang;Yun-guang Liu;Ren-guang Tang;Qun-qing Xu;Lan-qing Meng
近年来研究发现炎症与动脉粥样硬化的发病机制有关，而炎症基因是动脉粥样硬化发生的危险因素。动脉粥样硬化的早期阶段涉及从循环中募集炎性细胞及其跨内皮迁移。这一过程主要由细胞粘附分子介导。粘附分子P-选择素可能在动脉粥样硬化的发病机制中起一定作用。P-选择素基因多态性可能影响粘附分子的产生水平，与许多动脉粥样硬化性疾病有关。为了验证这一假设，我们调查了中国人群中P-选择素基因多态性与缺血性脑卒中的关系。我们采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和DNA测序方法，分析了305例缺血性脑卒中患者和280例年龄和性别匹配的对照者的P-选择素基因− 2，123 G/C、− 1，969 G/A、− 1，817 T/C和Thr 715 Pro的单核苷酸多态性。P-选择素基因多态性的基因型、等位基因和单倍型频率在缺血性脑卒中组和对照组之间差异无统计学意义。此外，在与缺血性卒中的任何亚型相关的任何多态性上，基因型、等位基因和单倍型均无显著关联。我们没有观察到P-选择素基因多态性与缺血性卒中或缺血性卒中的任何亚型之间的关联。然而，需要进一步的研究来探讨环境因素和P-选择素基因多态性在缺血性卒中风险中的复杂相互作用，特别是在不同种族的人群中。
Inflammation has recently proven to be associated with the pathogenesis of atherosclerosis and inflammatory genes are good candidates for the risk of developing atherosclerosis. The early phase of atherosclerosis involves the recruitment of inflammatory cells from the circulation and their transendothelial migration. This process is mainly mediated by cellular adhesion molecules. The adhesion molecule P-selectin may play a role in the pathogenesis of atherosclerosis. Polymorphism of P-selectin gene, which may affect the production level of the adhesion molecule, has been associated with a number of atherosclerotic disease. To test this hypothesis, we investigated the relationship of P-selectin gene polymorphisms and ischemic stroke in a Chinese population. We analyzed single nucleotide polymorphisms of P-selectin gene −2,123 G/C, −1,969 G/A, −1,817 T/C and Thr715Pro in three hundred and five patients with ischemic stroke and 280 age and sex matched controls, using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing method. There were no significant differences in the genotype, allele and haplotype frequencies of P-selectin gene polymorphisms between the group of patients with ischemic stroke and the control group. Furthermore, there was no significant association of genotype, allele and haplotype at any of the polymorphism in relation to any subtype of ischemic stroke. We did not observe an association between P-selectin gene polymorphisms and ischemic stroke or any subtype of ischemic stroke. However, further studies are needed to explore the complex interaction between environmental factors and P-selectin gene polymorphisms in the risk of ischemic stroke, particularly in ethnically different populations.