Effect of the 1BL.1RS translocation on the wheat endosperm, as revealed by proteomic analysis
Effect of the 1BL.1RS translocation on the wheat endosperm, as revealed by proteomic analysis
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DOI：
10.1002/pmic.200700488
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发表时间：
2007-12-01
期刊：
影响因子：
3.4
通讯作者：
Branlard, Gerard
中科院分区：
文献类型：
--
作者：
Gobaa, Samy;Bancel, Emmanuelle;Branlard, Gerard
将黑麦的1RS染色体引入小麦，使小麦对几种病原菌具有更强的抗性。今天，这种阻力已经被克服，但1BL。1RS易位仍然很有趣，因为它提高了产量，尽管它产生的流变性能较低。黑麦染色质渗入对小麦籽粒蛋白质组的影响尚未见报道。添加或不添加1BL的16个双单倍体系2-DE谱的比较。1RS易位，揭示了脯蛋白和其他胚乳蛋白的定量和定性变化。蛋白质。在有1BL的线条中发现了8个斑点。1 rs易位;另外16个点也从同一条线上消失了。两种基因型中均有12个位点符合上调或下调位点的标准。在易位基因型中，一个高度过表达的位点，被鉴定为具有9个半胱氨酸残基的γ -麦胶蛋白，表明LMW-GS缺乏是由1BL诱导的。1RS被一种相对相似的原蛋白的过度表达所抵消。此外，还有1BL中没有的一个点。1RS基因型为二聚体α -淀粉酶抑制剂。它被认为是解释与易位品种相关的粘性面团的一个有价值的候选者。
The introduction of the 1RS chromosome of rye into wheat made wheat more resistant to several pathogens. Today, this resistance has been overcome but the 1BL.1RS translocation remains interesting because of the improved yield and despite the lower rheological properties it produces. Nothing has been reported yet on the impact of rye chromatin introgression on the grain proteome of wheat. The comparison of the 2-DE profiles of 16 doubled haploid lines, with or without the 1BL.1RS translocation, revealed quantitative and qualitative proteic variations in prolamins and other endosperm. proteins. Eight spots were found specifically in lines having the 1BL.1RS translocation; 16 other spots disappeared from the same lines. Twelve spots, present in both genotypes, met the criteria for up- or down-regulated spots. In translocated genotypes, a highly overexpressed spot, identified as a gamma-gliadin with nine cysteine residues, suggests that the lack of LMW-GS induced by 1BL.1RS is counterbalanced by an overexpression of a relatively similar prolamin. Moreover, a spot that was absent from 1BL.1RS genotypes was identified as a dimeric alpha-amylase inhibitor. It was considered to be a valuable candidate to explain the sticky dough associated with translocated cultivars.