A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe.
A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe.
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DOI：
10.1016/s0021-9258(19)50688-3
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发表时间：
1992-02
期刊：
影响因子：
--
通讯作者：
Philippe Szankasi;Gerald;-R.;Smith
中科院分区：
文献类型：
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作者：
Philippe Szankasi;Gerald;-R.;Smith
在裂殖酵母粟酒裂殖酵母的减数分裂细胞中，DNA外切核酸酶活性在减数分裂前S期后增加约5倍，并降低至减数分裂前的初始水平。我们已将这种活性（指定为核酸外切酶 I）纯化至接近同质性。该活性与表观分子量为36,000的多肽共纯化。使用线性双链 DNA 底物，核酸外切酶 I 仅降解两端的 5' 端链，产生 3'-单链尾部。该酶还以相当的亲和力作用于带切口的环状 DNA。核酸外切酶 I 的减数分裂诱导及其作用模式与细菌中促进重组的核酸外切酶相似，表明核酸外切酶 I 参与了粟酒裂殖酵母的减数分裂同源重组。
In meiotic cells of the fission yeast Schizosaccharomyces pombe, a DNA exonuclease activity increased approximately 5-fold after premeiotic S-phase and decreased to the initial level before the meiotic divisions. We have purified this activity, designated exonuclease I, to near homogeneity. The activity co-purified with a polypeptide with an apparent molecular weight of 36,000. With a linear double-stranded DNA substrate, exonuclease I degraded only the 5‘-ended strand from each end to produce 3‘-single-stranded tails. The enzyme also acted on nicked circular DNA with comparable affinity. The meiotic induction of exonuclease I and its mode of action, similar to that of recombination-promoting exonucleases from bacteria, suggest that exonuclease I is involved in meiotic homologous recombination in S. pombe.