Transcriptome Profiling Reveals the Antitumor Mechanism of Polysaccharide from Marine Algae Gracilariopsis lemaneiformis.
Transcriptome Profiling Reveals the Antitumor Mechanism of Polysaccharide from Marine Algae Gracilariopsis lemaneiformis.
复制标题
转录组分析揭示了海藻龙须草多糖的抗肿瘤机制
DOI：
10.1371/journal.pone.0158279
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Xu N
中科院分区：
文献类型：
--
作者：
Kang Y;Li H;Wu J;Xu X;Sun X;Zhao X;Xu N
海藻是重要的生物质生产国之一，具有活性代谢产物，具有潜在的治疗肿瘤作用。红藻Gracilariopsis lemaneiformis(GP.Lemaneiformis)具有抗肿瘤活性，GP的多糖。龙血藤(PGL)已被证明是一种具有显著抗癌活性的成分。然而，PGL的抗癌作用机制尚不清楚。在本研究中，我们分析了PGL对3种人癌细胞株的生长抑制作用，发现PGL以时间和浓度依赖的方式抑制细胞增殖，降低细胞存活率，改变细胞形态。我们的转录组分析表明，PGL可以调控758个基因的表达，这些基因参与了细胞凋亡、细胞周期、核分裂和细胞死亡。此外，我们还证实了PGL诱导A549细胞凋亡和细胞周期停滞，并调节相关基因的表达。我们的工作为理解PGL对癌细胞的影响提供了一个框架，并可作为描绘GP抗肿瘤机制的资源。柳叶猴。
Seaweed is one of the important biomass producers and possesses active metabolites with potential therapeutic effects against tumors. The red alga Gracilariopsis lemaneiformis (Gp. lemaneiformis) possesses antitumor activity, and the polysaccharide of Gp. lemaneiformis (PGL) has been demonstrated to be an ingredient with marked anticancer activity. However, the anticancer mechanism of PGL remains to be elucidated. In this study, we analyzed the inhibitory effect of PGL on the cell growth of 3 human cancer cell lines and found that PGL inhibited cell proliferation, reduced cell viability, and altered cell morphology in a time- and concentration-dependent manner. Our transcriptome analysis indicates that PGL can regulate the expression of 758 genes, which are involved in apoptosis, the cell cycle, nuclear division, and cell death. Furthermore, we demonstrated that PGL induced apoptosis and cell cycle arrest and modulated the expression of related genes in the A549 cell line. Our work provides a framework to understand the effects of PGL on cancer cells, and can serve as a resource for delineating the antitumor mechanisms of Gp. lemaneiformis.