New insights into antibiotic resistome in drinking water and management perspectives: A metagenomic based study of small-sized microbes
New insights into antibiotic resistome in drinking water and management perspectives: A metagenomic based study of small-sized microbes
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DOI：
10.1016/j.watres.2018.12.069
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发表时间：
2019-04-01
期刊：
影响因子：
12.8
通讯作者：
Zhang, Tong
中科院分区：
文献类型：
--
作者：
Ma, Liping;Li, Bing;Zhang, Tong
饮用水中抗生素耐药基因(ARGs)的增殖及其对病原微生物的潜在水平转移可能导致抗生素失效。然而，饮用水中的抗菌素耐药性监测目前并不是常规做法。膜过滤消毒可能不能有效去除饮用水中ARGs的细菌宿主，特别是小型微生物，从而对人类健康构成威胁。本研究采用全基因组学方法，对中国大陆中国、香港和新加坡12个城市的20份生活饮用水样品进行了小型微生物(0.2-0.45亩)耐药性调查。共检测到265个ARG亚型，分属17个ARG类型，丰度从4.0×10(-2)拷贝/细胞到1.0×10(0)拷贝/细胞不等。耐药基因以多药耐药为主，杆菌素、氨基糖苷类耐药基因占主导地位，43种ARG亚型由小型微生物特异性携带。元基因组组装策略显示，产碱假单胞菌、铜绿假单胞菌和戈登分枝杆菌3种条件致病菌的片段分别携带mexW、aph(3‘)-I和aac(2’)-I。根据ARG、病原菌和携带ARG的病原菌的存在情况，将饮用水样品分为三组。对饮用水中小微生物抗生素耐药性的这些新见解表明，需要对饮用水供应进行更全面的ARGs监测和监测。这些发现，加上这项研究中提出的观点和战略，可以支持改善饮用水安全的倡议。(C)2019爱思唯尔有限公司。保留所有权利。
The proliferation of antibiotic resistance genes (ARGs) in drinking water and their potential horizontal transfer to pathogenic microbes may cause failure of antibiotics. However, antimicrobial resistome monitoring in drinking water is not currently routine. The bacterial hosts of ARGs, especially small-sized microbes in drinking water, may not be effectively removed by membrane filtration disinfection and thus pose threats to human health. In the present study, using metagenomic based approach, we investigated antibiotic resistome of small-sized microbes (0.2-0.45 mu m) in 20 household drinking water samples from 12 cities in Mainland China, Hong Kong and Singapore. A total of 265 ARG subtypes belonging to 17 ARG types were detected at abundances ranging from 4.0 x 10(-2) to 1.0 x 10(0) copies/cell. Multidrug, bacitracin and aminoglycoside resistance genes are dominant, and 43 ARG subtypes were specifically carried by small-size microbes. Metagenomic assembly strategy revealed fragments of three opportunistic pathogen, i.e. Pseudomonas alcaligenes, Pseudomonas aeruginosa and Mycobacterium gordonae, carried mexW, aph(3')-I and aac(2')-I, respectively. Drinking water samples were classified into three groups based on the presence of ARG, pathogen and ARG-carrying pathogen. These new insights into the antibiotic resistome of small microbes in drinking water over a broad scale indicate the need for more comprehensive ARGs monitoring and surveillance of drinking water supplies. These findings, together with the perspectives and strategies proposed in this study, could support initiatives to improve drinking water safety. (C) 2019 Elsevier Ltd. All rights reserved.