Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental metallic trace elements gradient: A microcosm study
Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental metallic trace elements gradient: A microcosm study
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DOI：
10.1016/j.scitotenv.2011.08.040
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发表时间：
2011-11-15
影响因子：
9.8
通讯作者：
Vandenbulcke, Franck
中科院分区：
文献类型：
--
作者：
Brulle, Franck;Lemiere, Sebastien;Vandenbulcke, Franck
两家冶炼厂（Metaleurop Nord 和 Nyrstar）过去的活动导致法国北部 Noyelles-Godault/Auby 地区表层土壤积累了大量金属微量元素 (TE)。将蚯蚓暴露于该地区收集的污染土壤中，以研究和更好地了解 TE 暴露引起的生理变化、适应机制和解毒。此前，我们已经从生态毒理学上重要的蚯蚓物种 Eisenia fetida 的免疫细胞中克隆和转录表征了潜在的生物标志物，这些蚯蚓在体内暴露于加有 TE 的标准土壤。在本研究中，在微环境暴露于以动力学方式表现出环境镉（Cd）梯度的天然土壤后，对臭艾球菌中四个候选指示基因（镉金属硫蛋白、辅肌动蛋白样蛋白、植物螯合素合酶和溶素）的表达动力学进行了分析。还测量了 TE 身体负担。这项微观研究提供了以下见解：(1) 4 个测试基因作为表达生物标志物的能力，(2) 通过所选基因的表达分析进行解毒过程，以及 (3) 土地利用对潜在生物标志物反应的影响（基因表达或 TE 吸收）。 (C) 2011 Elsevier B.V. 保留所有权利。
Past activities of 2 smelters (Metaleurop Nord and Nyrstar) led to the accumulation of high amounts of Metal Trace Elements (TEs) in top soils of the Noyelles-Godault/Auby area, Northern France. Earthworms were exposed to polluted soils collected in this area to study and better understand the physiological changes, the mechanisms of acclimation, and detoxification resulting from TE exposure. Previously we have cloned and transcriptionally characterized potential biomarkers from immune cells of the ecotoxicologically important earthworm species Eisenia fetida exposed in vivo to TE-spiked standard soils. In the present study, analysis of expression kinetics of four candidate indicator genes (Cadmium-metallothionein, coactosin like protein, phytochelatin synthase and lysenin) was performed in E. fetida after microcosm exposures to natural soils exhibiting an environmental cadmium (Cd) gradient in a kinetic manner. TE body burdens were also measured. This microcosm study provided insights into: (1) the ability of the 4 tested genes to serve as expression biomarkers, (2) detoxification processes through the expression analysis of selected genes, and (3) influence of land uses on the response of potential biomarkers (gene expression or TE uptake). (C) 2011 Elsevier B.V. All rights reserved.