Distinguishing mild cognitive impairment from Alzheimer’s disease with acrolein metabolites and creatinine in urine.
Distinguishing mild cognitive impairment from Alzheimer’s disease with acrolein metabolites and creatinine in urine.
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通过尿液中的丙烯醛代谢物和肌酐区分轻度认知障碍和阿尔茨海默病。
DOI：
10.1016/j.cca.2014.12.023
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M. et al.
中科院分区：
文献类型：
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作者：
Yoshida;M. et al.
我们之前报道过卒中后尿3-羟丙基巯基酸(3-HPMA)/肌酐（Cre）水平降低。本研究的目的是确定痴呆患者尿液中3-HPMA/Cre水平是否降低。方法采用LC-MS /MS法测定3-HPMA含量，ELISA法测定氨基酸共轭丙烯醛（AC-Acro）含量。该研究包括128名老年受试者，分为74名无痴呆（对照组）、22名轻度认知障碍（MCI）和32名阿尔茨海默病（AD）受试者。结果MCI合并AD组尿3-HPMA/Cre、AC-Acro/Cre显著低于对照组。此外，AD组尿Cre显著高于MCI组，AD组3-HPMA/Cre和AC-Acro/Cre显著低于MCI组。在这三个指标中，较低的3-HPMA/Cre比值与MMSE （Mini-Mental State Examination）的下降和CDRsob （Clinical Dementia Rating Scale Sum of Boxes Scores）的升高相关性最强。此外，痴呆受试者尿液中3-HPMA/Cre的降低与血浆中a - β40/42in的增加密切相关。结论3-HPMA/Cre是区分AD和MCI患者最可靠的生化指标。
BackgroundWe previously reported that the level of urinary 3-hydroxypropyl mercapturic acid (3-HPMA)/creatinine (Cre) was reduced following stroke. The aim of this study was to determine whether the level of 3-HPMA/Cre in urine was reduced in subjects with dementia.MethodsThe level of 3-HPMA was measured by LC–MS/MS, and that of amino acid conjugated acrolein (AC-Acro) was by ELISA. The study included 128 elderly subjects divided into 74 non-demented (control), 22 mild cognitive impairment (MCI) and 32 Alzheimer's disease (AD) subjects.ResultsThe urinary 3-HPMA/Cre and AC-Acro/Cre in MCI plus AD subjects were significantly lower than those in control subjects. In addition, urinary Cre in AD subjects was significantly higher than that in MCI subjects, and 3-HPMA/Cre and AC-Acro/Cre in AD subjects were significantly lower than that in MCI subjects. Among these three markers, the lower 3-HPMA/Cre ratio was most strongly correlated with the decline of MMSE (Mini-Mental State Examination) and the increase in CDRsob (Clinical Dementia Rating Scale Sum of Boxes Scores). Furthermore, reduction in 3-HPMA/Cre in urine was well correlated with increase in Aβ40/42in plasma in demented subjects.ConclusionThe results indicate that 3-HPMA/Cre in urine is the most reliable biochemical marker to distinguish AD subjects from MCI subjects among three markers.