PHOTOSTIMULATION USING CAGED GLUTAMATE REVEALS FUNCTIONAL CIRCUITRY IN LIVING BRAIN-SLICES
PHOTOSTIMULATION USING CAGED GLUTAMATE REVEALS FUNCTIONAL CIRCUITRY IN LIVING BRAIN-SLICES
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DOI：
10.1073/pnas.90.16.7661
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发表时间：
1993-08-15
影响因子：
11.1
通讯作者：
KATZ, LC
中科院分区：
文献类型：
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作者：
CALLAWAY, EM;KATZ, LC
一种对活体哺乳动物脑切片进行高空间分辨率功能电路映射的方法已经被开发出来。通过对切片高度受限区域（直径50-100微米）进行光刺激，同时保持对感兴趣的神经元的全细胞记录，可以揭示与单个神经元建立功能性突触连接的神经元的位置。光刺激是通过将脑切片浸泡在分子笼状的神经递质谷氨酸[l -谷氨酸-（4,5-二甲氧基-2-硝基苯基）酯]中来实现的，然后通过短脉冲将其转化为活性形式(
An approach for high-spatial-resolution mapping of functional circuitry in living mammalian brain slices has been developed. The locations of neurons making functional synaptic connections to a single neuron are revealed by photostimulation of highly restricted areas of the slice (50-100 mum in diameter) while maintaining a whole-cell recording of the neuron of interest. Photostimulation is achieved by bathing brain slices in a molecularly caged form of the neurotransmitter glutamate [L-glutamic acid alpha-(4,5-dimethoxy-2-nitrobenzyl) ester], which is then converted to the active form by brief pulses (