Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues
Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues
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DOI：
10.1007/s10534-011-9502-x
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发表时间：
2012-04
期刊：
影响因子：
3.5
通讯作者：
Jiu-li Zhang;Jin-Long Li;Xiao-dan Huang;Sun Bo;Wang Rihua;Shu Li;Shiwen Xu
中科院分区：
文献类型：
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作者：
Jiu-li Zhang;Jin-Long Li;Xiao-dan Huang;Sun Bo;Wang Rihua;Shu Li;Shiwen Xu
硒（Se）、硒蛋白N （SelN）和硒蛋白W （SelW）在肌肉疾病中起着至关重要的作用。硒水平高度调节硒蛋白mRNA水平。然而，很少有人尝试研究饲粮硒添加对鸟类肌肉SelN和SelW mRNA水平的影响。为研究硒对肌肉组织中SelN和SelW mRNA水平的调控作用，将1日龄雄性鸡分别饲喂1.0、2.0、3.0和5.0 mg/kg亚硒酸钠的商业饲粮和硒补充饲粮，为期90 d。收集肌肉组织（胸部、飞行、大腿、小腿和心肌），检测硒含量和SelN、SelW mRNA水平。分析硒代磷酸合成酶-1 （SPS-1）和硒代半胱氨酸合成酶（SecS） mRNA表达水平。硒处理显著提高了胸肌、大腿肌和小腿肌的SelN mRNA水平，其中以3.0 mg Se/kg饲粮的效果最大，但2.0 mg Se/kg饲粮的SelN mRNA水平最高。大腿和小腿肌肉中SelW mRNA丰度的变化对硒供应的反应增加。在达到最大水平后，高硒补充导致SelN和SelW mrna的减少。然而，SelN和SelW mRNA水平在不同骨骼肌和心肌中表现出不同的表达模式。由此可见，硒对骨骼肌和心肌SelN和SelW mRNA水平有高度调控作用，且不同肌肉组织对硒的敏感性存在差异。此外，硒补充还调节了SPS1和SecS mrna的水平。硒补充组SPS1和SecS mRNA表达水平升高。这些数据表明，硒调节SelN和SelW基因的表达，影响SecS和SPS1的mRNA水平。
Selenium (Se), selenoprotein N (SelN) and selenoprotein W (SelW) play a crucial role in muscle disorders. Se status highly regulates selenoprotein mRNA levels. However, few attempts have been performed on the effect of dietary Se supplementation on muscle SelN and SelW mRNA levels in birds. To investigate the effects of Se on the regulation of SelN and SelW mRNA levels in muscle tissues, one-day-old male chickens were fed either a commercial diet or a Se-supplemented diet containing 1.0, 2.0, 3.0 or 5.0 mg/kg sodium selenite for 90 days. Muscle tissues (breast, flight, thigh, shank and cardiac muscles) were collected and examined for Se content and mRNA levels of SelN and SelW. Moreover, Selenophosphate synthetase-1 (SPS-1) and selenocysteine-synthase (SecS) mRNA levels were analyzed. Significant increases in SelN mRNA levels were obtained in breast, thigh and shank muscles treated with Se, with maximal effects at 3.0 mg Se/kg diet, but 2.0 mg Se/kg diet resulted in peak levels of Sel N mRNA in flight muscles. Changes in SelW mRNA abundance in thigh and shank muscles increased in response to Se supply. After reaching a maximal level, higher Se supplementation led to a reduction in both SelN and SelW mRNAs. However, SelN and SelW mRNA levels displayed a different expression pattern in different skeletal and cardiac muscles. Thus, it suggested that skeletal and cardiac muscles SelN and SelW mRNA levels were highly regulated by Se supplementation and different muscle tissues showed differential sensitivity. Moreover, Se supplementation also regulated the levels of SPS1 and SecS mRNAs. The mRNA levels of SPS1 and SecS were enhanced in the Se supplemented groups. These data indicate that Se regulates the expression of SelN and SelW gene and affect the mRNA levels of SecS and SPS1.