Identification and characterization of 5 walnut MYB genes in response to drought stress involved in ABA signaling
Identification and characterization of 5 walnut MYB genes in response to drought stress involved in ABA signaling
复制标题
响应 ABA 信号传导的干旱胁迫的 5 个核桃 MYB 基因的鉴定和表征
DOI：
10.1007/s12298-021-01008-z
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发表时间：
2021-06-09
影响因子：
3.5
通讯作者：
Yang, Guiyan
中科院分区：
文献类型：
--
作者：
Li, Dapei;Peng, Shaobing;Yang, Guiyan
核桃是一种受欢迎的坚果树种，通常遭受干旱胁迫。然而，关于核桃对干旱胁迫的响应机制的信息很少，导致对其抗旱性缺乏基本的了解。为了挖掘更多能响应胁迫的功能基因，丰富核桃抗胁迫的理论基础，本研究从核桃中鉴定出5个具有完整orf的MYB基因，并利用qRT-PCR方法确认其基本生物信息、在不同组织中的表达模式以及对干旱和ABA胁迫的响应。结果表明，2个JrMYB基因属于R1-MYB亚家族，3个JrMYB基因属于R2R3-MYB亚家族，编码的蛋白长度为212 ~ 362 aa。系统发育分析将5个JrMYBs蛋白和40个拟南芥AtMYBs蛋白分为10个亚群。同一亚群的jrmyb在氨基酸序列的保守结构域和基序的组成以及DNA序列的外显子/内含子组织上表现出显著的相似性。qRT-PCR分析结果显示，JrMYB基因在不同组织中表达多样。干旱和ABA胁迫下，JrMYBs的表达量均显著上调或下调。最吸引人的是，与单独遭受干旱胁迫相比，干旱和额外的ABA处理大大提高了JrMYBs的转录水平。这些结果表明，JrMYB基因在植物生理过程和干旱以及ABA胁迫响应中发挥着重要作用，可能是ABA依赖性的植物干旱响应转录因子。
Walnut is a popular nut tree species and usually suffers from drought stress. However, little information is available on the mechanism of walnut responding to drought stress, resulting in lack of basic understanding for its resistance. In order to excavate more functional genes that can respond to stressors, and enrich the theoretical basis for walnut resistance, in this study, 5 MYB genes with complete ORFs were identified from J. regia and the basic bio-information as well as expression patterns in different tissues and response to drought and ABA stresses were confirmed using qRT-PCR assay. The results showed that 2 JrMYB genes belong to R1-MYB subfamily and 3 JrMYBs belong to R2R3-MYB, encoding the proteins from 212 to 362 aa in length. The phylogenetic analysis categorized proteins of 5 JrMYBs and 40 Arabidopsis AtMYBs into 10 subgroups. JrMYBs in the same subgroup exhibited significant similarities in the composition of conserved domains and motifs in amino acid sequences and exon/intron organization in DNA sequences. The results of qRT-PCR analysis revealed that JrMYB genes diversely expressed in various tissues. Moreover, the expression values of JrMYBs were upregulated or downregulated significantly under drought and ABA stresses. Most attractively, in contrast with suffering from drought stress alone, the treatments with drought and additional ABA greatly enhanced the transcript levels of JrMYBs. All these results suggested that JrMYB genes play a vital role in plant biological processes and drought as well as ABA stress response, and possibly perform as ABA-dependent drought response transcription factors in plant.