Constitutive overexpression of the TaNF-YB4 gene in transgenic wheat significantly improves grain yield.
Constitutive overexpression of the TaNF-YB4 gene in transgenic wheat significantly improves grain yield.
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DOI：
10.1093/jxb/erv370
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发表时间：
2015-11
影响因子：
6.9
通讯作者：
Lopato S
中科院分区：
文献类型：
--
作者：
Yadav D;Shavrukov Y;Bazanova N;Chirkova L;Borisjuk N;Kovalchuk N;Ismagul A;Parent B;Langridge P;Hrmova M;Lopato S
与野生型植物相比，在转基因小麦中组成型过表达NF-YB导致谷粒产量增加20-30%（cv. Gladius）在种子大小或数量上没有负面变化。异源三聚体核因子Y（NF-Ys）在所有真核生物中参与各种生命功能的调节。尽管许多NF-Y亚基已经在模式植物中被表征，但只有少数在作物中进行了功能评估。本研究从耐旱小麦（Triticum aestivum L.）CV. RAC 875），并研究了TaNF-YB 4在澳大利亚小麦栽培品种Gladius中过表达的影响。TaNF-YB 4是作为两个连续酵母双杂交（Y2 H）筛选的结果分离的，其中ZmNF-YB 2a用作起始诱饵。在第一次Y2 H筛选中分离了一种新的NF-YC亚基，命名为TaNF-YC 15，并在第二次筛选中用作诱饵，第二次筛选鉴定了两种小麦NF-YB亚基，TaNF-YB 2和TaNF-YB 4。TaNF-YB 2/TaNF-YC 15二聚体的三维建模揭示了可能是相互作用选择性基础的结构决定因素。将TaNF-YB 4基因置于来自玉米的强组成型多聚泛素启动子的控制下，并通过基因枪轰击将其导入小麦。在充分浇水条件下（T1-T3代）和轻度干旱条件下（T2代）评估了具有上调水平的TaNF-YB 4的几个独立的转基因株系的生长和产量组成。在接近田间试验的条件下，在大的深容器中进行T2植物的分析。在最佳浇水条件下，转基因小麦植株产生了显着更多的穗，但其他产量构成因素没有改变。与未转化的对照植物相比，这导致谷物产量增加20-30%。在水分限制条件下，转基因株系在产量表现上保持平价。
Constitutive overexpression of NF-YB in transgenic wheat leads to a 20–30% increase in grain yield compared with wild-type plants (cv. Gladius) without negative changes in seed size or number. Heterotrimeric nuclear factors Y (NF-Ys) are involved in regulation of various vital functions in all eukaryotic organisms. Although a number of NF-Y subunits have been characterized in model plants, only a few have been functionally evaluated in crops. In this work, a number of genes encoding NF-YB and NF-YC subunits were isolated from drought-tolerant wheat (Triticum aestivum L. cv. RAC875), and the impact of the overexpression of TaNF-YB4 in the Australian wheat cultivar Gladius was investigated. TaNF-YB4 was isolated as a result of two consecutive yeast two-hybrid (Y2H) screens, where ZmNF-YB2a was used as a starting bait. A new NF-YC subunit, designated TaNF-YC15, was isolated in the first Y2H screen and used as bait in a second screen, which identified two wheat NF-YB subunits, TaNF-YB2 and TaNF-YB4. Three-dimensional modelling of a TaNF-YB2/TaNF-YC15 dimer revealed structural determinants that may underlie interaction selectivity. The TaNF-YB4 gene was placed under the control of the strong constitutive polyubiquitin promoter from maize and introduced into wheat by biolistic bombardment. The growth and yield components of several independent transgenic lines with up-regulated levels of TaNF-YB4 were evaluated under well-watered conditions (T1–T3 generations) and under mild drought (T2 generation). Analysis of T2 plants was performed in large deep containers in conditions close to field trials. Under optimal watering conditions, transgenic wheat plants produced significantly more spikes but other yield components did not change. This resulted in a 20–30% increased grain yield compared with untransformed control plants. Under water-limited conditions transgenic lines maintained parity in yield performance.