Tocotrienols Suppress Proinflammatory Markers and Cyclooxygenase-2 Expression in RAW264.7 Macrophages
Tocotrienols Suppress Proinflammatory Markers and Cyclooxygenase-2 Expression in RAW264.7 Macrophages
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DOI：
10.1007/s11745-009-3326-2
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发表时间：
2009-09-01
期刊：
影响因子：
1.9
通讯作者：
Nesaretnam, Kalanithi
中科院分区：
文献类型：
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作者：
Yam, Mun-Li;Hafid, Sitti Rahma Abdul;Nesaretnam, Kalanithi
生育三烯酚是强效的断链抗氧化剂。此外，现在已知它们表现出各种非抗氧化特性，如抗癌，神经保护和降胆固醇功能。本研究旨在研究富含生育三烯酚的组分（TRF）和单个生育三烯酚异构体（即δ-、γ-和α-生育三烯酚）对脂多糖刺激的RAW 264.7巨噬细胞的抗炎作用。广泛研究的维生素E形式，α-生育酚，被用作比较。用脂多糖刺激RAW 264.7诱导各种炎症标志物的释放。10 μ g/ml的TRF和所有生育三烯酚亚型均显着抑制白细胞介素-6和一氧化氮的产生。然而，只有α-生育三烯酚在降低肿瘤坏死因子-α产生方面表现出显著效果。除γ-生育三烯酚外，TRF和所有生育三烯酚异构体均减少前列腺素E-2的释放。除α-生育酚外，所有维生素E形式都伴随着环氧合酶-2基因表达的下调。总的来说，数据表明生育三烯酚是比α-生育酚更好的抗炎剂，最有效的形式是δ-生育三烯酚。
Tocotrienols are powerful chain breaking antioxidant. Moreover, they are now known to exhibit various non-antioxidant properties such as anti-cancer, neuroprotective and hypocholesterolemic functions. This study was undertaken to investigate the anti-inflammatory effects of tocotrienol-rich fraction (TRF) and individual tocotrienol isoforms namely delta-, gamma-, and alpha-tocotrienol on lipopolysaccharide-stimulated RAW264.7 macrophages. The widely studied vitamin E form, alpha-tocopherol, was used as comparison. Stimulation of RAW264.7 with lipopolysaccharide induced the release of various inflammatory markers. 10 mu g/ml of TRF and all tocotrienol isoforms significantly inhibited the production of interleukin-6 and nitric oxide. However, only alpha-tocotrienol demonstrated a significant effect in lowering tumor necrosis factor-alpha production. Besides, TRF and all tocotrienol isoforms except gamma-tocotrienol reduced prostaglandin E-2 release. It was accompanied by the down-regulation of cyclooxygenase-2 gene expression by all vitamin E forms except alpha-tocopherol. Collectively, the data suggested that tocotrienols are better anti-inflammatory agents than alpha-tocopherol and the most effective form is delta-tocotrienol.