Sex-specific DoublesexM expression in subsets of Drosophila somatic gonad cells.
Sex-specific DoublesexM expression in subsets of Drosophila somatic gonad cells.
复制标题
果蝇体形性腺细胞子集中的性别特异性双触及表达。
DOI：
10.1186/1471-213x-7-113
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发表时间：
2007-10-12
影响因子：
--
通讯作者：
Oliver, Brian
中科院分区：
文献类型：
--
作者：
Hempel, Leonie U.;Oliver, Brian
在黑腹果蝇中，前mRNA剪接层次控制性别身份，并最终导致性别特异性Doublyex（DSX）转录因子亚型。雄性特异性DSXM抑制参与雌性发育的基因并激活参与雄性发育的基因。dsx在胚胎发生过程中的空间和时间控制还没有很好的记录。在这里，我们表明，DSXM是专门表达的男性体细胞性腺细胞在胚胎发生的子集。在睾丸形成后，生殖细胞保持与DSXM表达细胞接触，包括枢纽细胞和减数分裂前体细胞囊肿细胞，其在幼虫和成年睾丸中的精子发生期间围绕生殖细胞。我们发现，dsx的转录调控，除了在前mRNA剪接水平的性别决定层次的调节。dsx位点在空间上受体细胞性腺特性的控制。DSXM在接触生殖细胞的细胞中的持续表达表明体细胞性别决定途径对生殖细胞发育的持续短期影响。
In Drosophila melanogaster, a pre-mRNA splicing hierarchy controls sexual identity and ultimately leads to sex-specific Doublesex (DSX) transcription factor isoforms. The male-specific DSXM represses genes involved in female development and activates genes involved in male development. Spatial and temporal control of dsx during embryogenesis is not well documented. Here we show that DSXM is specifically expressed in subsets of male somatic gonad cells during embryogenesis. Following testis formation, germ cells remain in contact with DSXM-expressing cells, including hub cells and premeiotic somatic cyst cells that surround germ cells during spermatogenesis in larval and adult testes. We show that dsx is transcriptionally regulated in addition to being regulated at the pre-mRNA splicing level by the sex determination hierarchy. The dsx locus is spatially controlled by somatic gonad identity. The continuous expression of DSXM in cells contacting the germline suggests an ongoing short-range influence of the somatic sex determination pathway on germ cell development.