Stability and metabolism of Arbutus unedo bioactive compounds (phenolics and antioxidants) under in vitro digestion and colonic fermentation
Stability and metabolism of Arbutus unedo bioactive compounds (phenolics and antioxidants) under in vitro digestion and colonic fermentation
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DOI：
10.1016/j.foodchem.2016.01.076
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发表时间：
2016-06-15
期刊：
影响因子：
8.8
通讯作者：
Motilva, Maria-Jose
中科院分区：
文献类型：
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作者：
Mosele, Juana I.;Macia, Alba;Motilva, Maria-Jose
杨梅的天然抗氧化剂凸显了这种水果作为生物活性化合物天然来源的重要性。在本研究中，为了评估酚类化合物、抗坏血酸和脂溶性抗氧化剂（α-生育酚、β-胡萝卜素和叶黄素）的稳定性，对A. unedo果实进行了体外胃肠消化。之后，将不可吸收的部分与人类粪便进行厌氧培养，并提出了来自A. unedo果实的棓单宁、鞣花单宁、黄烷-3-醇和花青素的代谢途径。结果表明，水果中果胶的存在阻碍了酚类化合物（没食子酸和鞣花酸除外）和脂溶性维生素在胃消化过程中的溶解。十二指肠消化过程中果胶凝胶的降解有利于酚类化合物和脂溶性抗氧化剂向介质中的释放。人类微生物群的分解代谢活动导致多种简单酚类的产生，例如对羟基苯甲酸和儿茶酚，它们源自棓单宁、鞣花单宁、3-黄烷醇和花青素的分解代谢。 (C) 2016 Elsevier Ltd. 保留所有权利。
The natural antioxidants of Arbutus unedo highlight the importance of this fruit as natural source of bioactive compounds. In the present study, to evaluate the stability of phenolic compounds, ascorbic acid and fat-soluble antioxidants (alpha-tocopherol, beta-carotene and lutein), in vitro gastrointestinal digestion was applied to A. unedo fruit. After that, the non-absorbable fraction was anaerobically incubated with human faeces and the metabolic pathway for gallotannins, ellagitannins, flavan-3-ols and anthocyanins from A. unedo fruit was proposed. The results showed that the presence of pectin from the fruit hampered the solubilization of the phenolic compounds (with exception of gallic and ellagic acids) and fat-soluble vitamins during gastric digestion. Degradation of pectin-gel during the duodenal digestion favored the release of the phenolic compounds and fat-soluble antioxidants to the media. The catabolic activity of human microbiota led to the generation of a wide range of simple phenols, such as p-hydroxybenzoic acid and catechol, derived from the catabolism of gallotannins, ellagitannins, flavan-3-ols and anthocyanins. (C) 2016 Elsevier Ltd. All rights reserved.