The Female Encounter Test: A Novel Method for Evaluating Reward-Seeking Behavior or Motivation in Mice.
The Female Encounter Test: A Novel Method for Evaluating Reward-Seeking Behavior or Motivation in Mice.
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DOI：
10.1093/ijnp/pyv062
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发表时间：
2015-05-29
期刊：
影响因子：
--
通讯作者：
Matsuda T
中科院分区：
文献类型：
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作者：
Ago Y;Hasebe S;Nishiyama S;Oka S;Onaka Y;Hashimoto H;Takuma K;Matsuda T
动机降低是精神疾病的一个重要标志，包括抑郁症。我们描述了雌性遭遇测试，一种评价小鼠奖赏寻求行为的新方法。试验装置由三个开放室组成，形成有隔板，允许动物从一个室自由移动到另一个室。使测试雄性小鼠在该装置中适应，随后将雌性和雄性小鼠分别引入左室和右室中的丝网盒中。测量测试雄性小鼠在雌性或雄性区域中花费的时间10分钟。所有六种测试的小鼠都表现出对雌性遭遇的明显偏好。在7-30周龄小鼠中观察到偏好。的偏好被封闭的去势的居民男性测试小鼠，并没有受到影响的阶段的月经周期的女性入侵者。这种偏好在抑郁症小鼠模型中受损，包括社会隔离饲养、皮质酮治疗和脂多糖治疗的小鼠。氟伏沙明减轻了隔离饲养和脂多糖治疗的小鼠的损害，并改善了代谢型谷氨酸2/3受体拮抗剂LY 341495皮质酮治疗的小鼠。与雌性而非雄性小鼠相遇增加了测试雄性小鼠的丘脑核壳中的c-Fos表达。此外，多巴胺D1和D2受体拮抗剂可阻断c-Fos表达的偏好和多巴胺诱导的增加。这些研究结果表明，成年雄性小鼠的动机可以很容易地通过量化女性的遭遇来评估。
Reduced motivation is an important marker of psychiatric disorders, including depression. We describe the female encounter test, a novel method of evaluating reward-seeking behavior in mice. The test apparatus consists of three open chambers, formed with partitions that allow the animal to move freely from one chamber to another. A test male mouse is habituated in the apparatus, and subsequently a female and male mouse are introduced into a wire-mesh box in the left and right chamber, respectively. The time the test male mouse spends in the female or male area is measured for 10min. All six strains of mice tested showed a significant preference for female encounters. The preference was observed in 7–30-week-old mice. The preference was blocked by castration of the resident male test mouse, and was not affected by the phase of the menstrual cycle of the female intruder. The preference was impaired in mouse models of depression, including social isolation-reared, corticosterone-treated, and lipopolysaccharide-treated mice. The impairment was alleviated by fluvoxamine in isolation-reared and lipopolysaccharide-treated mice, and it was improved by the metabotropic glutamate 2/3 receptor antagonist LY341495 in corticosterone-treated mice. Encounter with a female, but not male, mouse increased c-Fos expression in the nucleus accumbens shell of test male mice. Furthermore, both the preference and encounter-induced increases in c-Fos expression were blocked by dopamine D1 and D2 receptor antagonists. These findings indicate that motivation in adult male mice can be easily evaluated by quantitating female encounters.