Linking key steps of microRNA biogenesis by TREX-2 and the nuclear pore complex inArabidopsis
Linking key steps of microRNA biogenesis by TREX-2 and the nuclear pore complex inArabidopsis
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DOI：
10.1038/s41477-020-0726-z
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发表时间：
2020-07-20
期刊：
影响因子：
18
通讯作者：
Chen, Xuemei
中科院分区：
文献类型：
--
作者：
Zhang, Bailong;You, Chenjiang;Chen, Xuemei
这项研究表明，植物中的TREX-2复合体协调miRNA生物合成中的转录、加工和输出步骤，并与核孔蛋白NUP 1共同参与miRNA的输出。miRNA生物发生的主要步骤是否或如何协调在很大程度上是未知的。在这里，我们表明植物TREX-2复合物促进miRNA生物合成的多个步骤，包括转录，加工和核输出。TREX-2的核心亚基THP 1和SAC 3A与RNA聚合酶II相互作用并共定位，促进核质中MIRs基因的转录。TREX-2与微处理器组件SERRATE相互作用，促进核质中切割体的形成。THP 1还与核膜上的核孔蛋白NUP 1相互作用并共定位。NUP 1和THP 1促进miRNA和ARGONAUTE 1的核输出。这些结果表明，TREX-2协调miRNA生物发生中的转录、加工和输出步骤，以确保有效的miRNA生产。
This study shows that theArabidopsisTREX-2 complex coordinates the transcription, processing and export steps in miRNA biogenesis, and that it acts with the nucleoporin NUP1 in miRNA export.Unlike in metazoans, the stepwise biogenesis of microRNAs (miRNAs) in plants occurs within the nucleus. Whether or how the major steps in miRNA biogenesis are coordinated is largely unknown. Here we show that the plant TREX-2 complex promotes multiple steps in miRNA biogenesis, including transcription, processing and nuclear export. THP1 and SAC3A-the core subunits of TREX-2-interact and colocalize with RNA polymerase II to promote the transcription ofMIRgenes in the nucleoplasm. TREX-2 interacts with the microprocessor component SERRATE and promotes the formation of dicing bodies in the nucleoplasm. THP1 also interacts and colocalizes with the nucleoporin protein NUP1 at the nuclear envelope. NUP1 and THP1 promote the nuclear export of miRNAs and ARGONAUTE1. These results suggest that TREX-2 coordinates the transcription, processing and export steps in miRNA biogenesis to ensure efficient miRNA production.