Multiple tissue transformation in adult zebrafish by gene gun bombardment and muscular injection of naked DNA
Multiple tissue transformation in adult zebrafish by gene gun bombardment and muscular injection of naked DNA
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DOI：
10.1007/s101260000056
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发表时间：
2001-03-01
影响因子：
3
通讯作者：
Gong, ZY
中科院分区：
文献类型：
--
作者：
Sudha, PM;Low, S;Gong, ZY
利用人巨细胞病毒（HCMV）启动子驱动的绿色荧光蛋白（GFP）报告基因，研究了基因枪（或粒子轰击）和肌肉注射两种直接基因转移方法在斑马鱼体内转化成年组织的效率。粒子轰击成年斑马鱼引起的内化和表达的质粒仅在表层，如上皮细胞，色素细胞，内皮细胞和神经元，而直接注射主要转化的几个束附近或周围的注射部位的肌纤维。在几种非肌肉组织中表达也很明显，如皮肤上皮细胞、色素细胞、血管细胞和神经元样细胞。两种方法的GFP表达持续超过50天。这些观察结果表明，这些方法的组织特异性启动子的功能分析，DNA疫苗的交付，和肌肉表达的其他有用的基因的潜力。
The efficiency of two direct gene transfer methods, gene gun (or particle bombardment) and intramuscular injection, in transforming adult zebrafish tissues in vivo was examined by a noninvasive approach using green fluorescent protein (GFP) reporter gene driven by the ubiquitously expressed human cytomegalovirus promoter. Particle bombardment of adult zebrafish caused internalization and expression of the plasmid only in the superficial layer such as epithelial cells, pigment cells, endothelial cells, and neurons, whereas direct injection primarily transformed muscle fibers of several bundles near or around the injection site. Expression was also evident in several nonmuscle tissues, such as skin epithelia, pigment cells, blood vessel cells, and neuron-like cells. GFP expression persisted for more than 50 days with both methods. These observations indicate the potential of these methods for functional analysis of tissue-specific promoters, delivery of DNA vaccine, and muscular expression of other useful genes.