Fractionation of polymeric procyanidins from lowbush blueberry and quantification of procyanidins in selected foods with an optimized normal-phase HPLC-MS fluorescent detection method
Fractionation of polymeric procyanidins from lowbush blueberry and quantification of procyanidins in selected foods with an optimized normal-phase HPLC-MS fluorescent detection method
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DOI：
10.1021/jf020214v
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发表时间：
2002-08-14
影响因子：
6.1
通讯作者：
Prior, RL
中科院分区：
文献类型：
--
作者：
Gu, LW;Kelm, M;Prior, RL
在 Sephadex LH-20 柱上从低丛蓝莓中分离出聚合原花青素。通过硫解测定聚合物的聚合度(DP)在19.9至114.1的范围内。正相HPLC分析表明，聚合原花青素不含DIP < 10的低聚原花青素。聚合物在色谱图末尾作为单峰洗脱。修改正相 HPLC 梯度，以改善原花青素单体通过十聚体的分离，并洗脱除明显峰之外的所有聚合物。单体到十聚体均单独定量。使用纯化聚合物的混合物作为外标对所有聚合物（DP > 10）进行定量。研究发现，聚合物是棕色高粱麸皮、蔓越莓和蓝莓中的主要原花青素。聚合物峰的硫解表明表儿茶素作为延伸单元存在于这些食品中，然而，儿茶素和表儿茶素之间末端单元的组成差异很大，或者在蔓越莓的情况下为A型二聚体连接。
The polymeric procyanidins were fractionated from lowbush blueberry on a Sephadex LH-20 column. The degree of polymerization (DP) for the polymers was determined by thiolysis to be in a range of 19.9 to 114.1. Normal-phase HPLC analysis indicated that the polymeric procyanidins did not contain oligomeric procyanidins with DIP < 10. The polymers eluted as a single peak at the end of the chromatogram. The normal-phase HPLC gradient was modified to improve the separation of procyanidin monomers through decamers and to elute all the polymers beyond those as a distinct peak. Monomers through decamers were quantified individually. All the polymers (DP > 10) were quantified using a mixture of purified polymers as an external standard. Polymers were found to be the dominant procyanidins in brown sorghum bran, cranberry, and blueberry. Thiolysis of the polymer peaks indicated that epicatechin was present as extension units in these foods, however, the composition of terminal units varied considerably between catechin and epicatechin, or an A-type dimer linkage in the case of cranberry.