Lipid droplets in the pheromone gland of wild silkmoth <i>Bombyx mandarina</i>
Lipid droplets in the pheromone gland of wild silkmoth <i>Bombyx mandarina</i>
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野蚕蛾信息素腺中的脂滴<i>Bombyx mandarina</i>
DOI：
10.11416/jibs.87.2_029
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发表时间：
2018
影响因子：
--
通讯作者：
Kanzaki Ryohei
中科院分区：
文献类型：
--
作者：
Fujii Takeshi;Sakurai Takeshi;Ito Katsuhiko;Yokoyama Takeshi;Kanzaki Ryohei
家蚕酚是从家蚕中化学鉴定出的第一种性信息素。到目前为止，桑蚕醇在家蚕信息素腺体(PG)中的生物合成机制已经得到了深入的研究。家蚕信息素产生细胞最显著的特征是细胞质中有丰富的脂滴。在性信息素的生物合成过程中，PG中的LDS被认为是信息素前体的脂肪储存器官。然而，除家蚕外的其他蛾种中LDS的存在仍然很少被描述。因此，我们推测家蚕PG中LD的形成可能是由于通过杂交产生体型较大的商业品系而失去飞行而在驯化条件下产生的肥大所致。为了验证我们的假设，我们研究了一个密切相关的物种--野大麦的PG中的LDS。大黄鱼的明场显微镜观察发现了直径约为10μm的候选LDS。荧光显微镜显示这些候选LDS可以被尼罗红染色。随后的激光扫描共聚焦显微镜观察证实，这些候选LDS定位于野桑蛾PG的性信息素产生细胞的细胞质中，表明这些候选LDS是LDS。因此，我们得出结论：家蚕和野桑蚕之间LD的形成是一个保守的性状，家蚕LD的形成可能不是驯化引起的。
Bombykol is the first sex pheromone that was chemically identified from silkmoth Bombyx mori. To date, the biosynthetic mechanism of bombykol in the pheromone gland (PG) of B. mori has been intensively investigated. The most prominent characteristic of pheromone-producing cells in B. mori is the abundance of cytoplasmic lipid droplets (LDs). During sex pheromone biosynthesis, LDs in the PG have been implicated to play important roles as lipid storage organs for the pheromone precursors. However, the presence of LDs in other moth species except for B. mori remains poorly described. Thus, we hypothesized that LD formation in the PG of B. mori may be caused by corpulence under domestication resulting from the loss of flight through hybridization for generating commercial lines with enlarged body size. To verify our hypothesis, we explored LDs in the PG of a closely related species, B. mandarina. A bright-field microscopic observation of B. mandarina PG revealed the presence of candidate LDs with a diameter of approximately 10 μm. Fluorescent microscopy revealed that these candidate LDs could be stained with a Nile red. Subsequent observation under a confocal laser-scanning microscopy confirmed that these candidate LDs were localized in the cytoplasm of the sex pheromone-producing cells in the PG of B. mandarina, indicating that these candidates were LDs. Therefore, we concluded that LD formation is a conserved trait between B. mori and B. mandarina, and the formation of LDs in B. mori may be not caused by domestication.