A New Lipid Anchor for Sparsely Tethered Bilayer Lipid Membranes
A New Lipid Anchor for Sparsely Tethered Bilayer Lipid Membranes
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DOI：
10.1021/la8033275
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发表时间：
2009-04-07
期刊：
影响因子：
3.9
通讯作者：
Losche, Mathias
中科院分区：
文献类型：
--
作者：
Heinrich, Frank;Ng, Tiffany;Losche, Mathias
研究了β-巯基乙醇和新合成脂质1，2-二棕榈基-3 [omega-巯基壬基（环氧乙烷）]甘油（FC 16）的混合自组装单分子膜（SAM）形成由各种磷脂完成的稀疏束缚双层脂质膜（stBLM）的能力。我们研究了基于FC 16的stBLM的结构和功能特性，并将其与使用先前表征的合成脂质1，2-二肉豆蔻基-3-[ω-巯基六（环氧乙烷）]甘油（WC 14）制备的stBLM进行了比较。基于FC 16的stBLM显示出对离子转移的增加的电阻率和类似于0.5rim的膜下空间的增加。重要的是，基于FC 16的stBLM与带电荷的磷脂如1-棕榈酰基-2-油酰基-sn-甘油基-3-磷酸甘油（POPG）形成明确的完整双层。在这些研究中，POPG以与浸没溶液中相同的比例掺入外层单层瓣叶中，但被排除在内层瓣叶之外。在我们迄今为止研究的所有情况下，双层内脂质的面积密度平均接近于游离双层膜中的面积密度。对于带电荷的磷脂，FC 16似乎提供了一个明显的优势超过WC 14的形成明确的stBLM。
Mixed self-assembled monolayers (SAMs) of beta-mercaptoethanol and the new synthetic lipid 1,2-dipalmityl-3[omega-mercaptonona(ethylene oxide)] glycerol (FC16) were investigated for their ability to form sparsely tethered bilayer lipid membranes (stBLMs) completed with various phospholipids. We investigated the structural and functional properties of FC16-based stBLMs and compared these to stBLMs prepared using a previously characterized synthetic lipid, 1,2-dimyristyl-3-[omega-mercaptohexa(ethylene oxide)] glycerol (WC14). FC16-based stBLMs show increased resistivity to ion transfer and an increase in the submembrane space of similar to 0.5 rim. Importantly, FC16-based stBLMs formed well-defined, complete bilayers with charged phospholipids such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG). In these, POPG incorporates into the outer monolayer leaflet in the same ratio as in the immersion solution but is excluded from the inner leaflet. In all cases that we have investigated thus far, the area densities of the lipids within the bilayers were on average close to those in free bilayer membranes. For charged phospholipids, FC16 appears to provide a distinct advantage over WC14 for the formation of well-defined stBLMs.