Epsin: Inducing membrane curvature
Epsin: Inducing membrane curvature
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DOI：
10.1016/j.biocel.2006.12.004
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发表时间：
2007-01-01
影响因子：
4
通讯作者：
De Wolf, Marc J. S.
中科院分区：
文献类型：
--
作者：
Horvath, Caroline A. J.;Vanden Broeck, Davy;De Wolf, Marc J. S.
Epsin 最初是通过与另一种辅助蛋白 Eps 15 结合而发现的。epsin 家族的成员作为辅助蛋白在网格蛋白介导的内吞作用中发挥着重要作用。尽管所有的epsin本质上都有助于膜变形，但已经描述了epsin同工型在细胞内作用位点和/或组织分布方面有所不同。除了诱导膜弯曲外，epsin 还发挥着作为衔接蛋白的关键功能，偶联网格蛋白辅助摄取的各种成分，并在选择和识别货物中发挥重要作用。此外，epsin 具有阻止有丝分裂期间囊泡形成的能力。为了执行所有这些功能，epsin 除了通过其 ENTH 结构域与 PtdIns(4,5)P-2 相互作用外，还与网格蛋白介导的内吞系统的不同成分进行多种蛋白质相互作用。最近，RNA干扰已成功地用于产生组成型沉默epsin表达的细胞系，该细胞系可用于研究多个配体的内化。 (c) 2007 Elsevier Ltd. 保留所有权利。
Epsin was originally discovered by virtue of its binding to another accessory protein, Eps 15. Members of the epsin family play an important role as accessory proteins in clathrin-mediated endocytosis. Epsin isoforms have been described that differ in intracellular site of action and/or in tissue distribution, although all epsins essentially contribute to membrane deformation. Besides inducing membrane curvature, epsin also plays a key function as adaptor protein, coupling various components of the clathrin-assisted uptake and fulfils an important role in selecting and recognizing cargo. Furthermore, epsin possesses the ability to block vesicle formation during mitosis. To perform all these functions, epsin, apart from interacting with PtdIns(4,5)P-2 via its ENTH domain, also engages in several protein interactions with different components of the clathrin-mediated endocytic system. Recently, RNA interference has successfully been exploited to generate a cell line constitutively silencing epsin expression, which can be used to study internalization of multiple ligands. (c) 2007 Elsevier Ltd. All rights reserved.