Biomarkers screening between preoperative and postoperative patients in pancreatic cancer.
Biomarkers screening between preoperative and postoperative patients in pancreatic cancer.
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DOI：
10.7314/apjcp.2013.14.7.4161
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发表时间：
2013-07
期刊：
影响因子：
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通讯作者：
Pei Li;Juan Yang;Qingyong Ma;Zheng Wu;Chen Huang;Xuqi Li;Z. Wang
中科院分区：
文献类型：
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作者：
Pei Li;Juan Yang;Qingyong Ma;Zheng Wu;Chen Huang;Xuqi Li;Z. Wang
OBJECTIVE To investigate discriminating protein patterns and potential biomarkers in serum samples between pre/postoperative pancreatic cancer patients and healthy controls. METHODS 23 serum samples from PC patients (12 preoperative and 11 postoperative) and 76 from healthy controls were analyzed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique combined with magnetic beads-based weak cation-exchange chromatography (MB-WCX). ClinProTools software selected several markers that made a distinction between pancreatic cancer patients and healthy controls. RESULTS 49 m/z distinctive peaks were found among the three groups, of which 33 significant peaks with a P < 0.001 were detected. Two proteins could distinguish the preoperative pancreatic cancer patients from the healthy controls. About 15 proteins may be potential biomarkers in assessment of pancreatic cancer resection. CONCLUSION MB-MALDI-TOF-MS method could generate serum peptidome profiles of pancreatic cancer and provide a new approach to identify potential biomarkers for diagnosis and prognosis of this malignancy.
