Purification of a novel peptide derived from Mytilus coruscus and in vitro/in vivo evaluation of its bioactive properties
Purification of a novel peptide derived from Mytilus coruscus and in vitro/in vivo evaluation of its bioactive properties
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DOI：
10.1016/j.fsi.2013.01.013
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发表时间：
2013-05-01
影响因子：
4.7
通讯作者：
Park, Pyo-Jam
中科院分区：
文献类型：
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作者：
Kim, Eun-Kyung;Oh, Hyun-Jung;Park, Pyo-Jam
过量的氧化剂可促进炎症反应。此外，伴随氧化应激的慢性炎症与许多疾病的各个步骤有关。从这方面来看，我们研究了一种抗氧化肽，以防止氧化过度表达的炎症反应。为了制备该肽，采用8种蛋白酶进行酶解，并利用电子自旋共振（ESR）光谱法研究了水解产物的抗氧化性能。木瓜蛋白酶水解物具有明显的自由基清除活性，用连续色谱法进一步纯化。最终获得了一种新的抗氧化肽，其序列为Ser-Leu-Pro-Ile-Gly-Leu-Met-Ile-Ala-Met。在硫代巴比妥酸活性物质（TBARS）实验中，口服该肽可有效抑制丙二醛（MDA）水平，并在超氧化物歧化酶（SOD）和谷胱甘肽-s转移酶（GST）实验中证实其抗氧化酶活性。这是首次报道从贻贝水解液中提取的抗氧化肽，这些结果也表明该肽具有强大的抗氧化活性，并可能增强抗炎反应。(C) 2013 Elsevier Ltd.版权所有。
Excess oxidant can promote inflammatory responses. Moreover, chronic inflammation accompanied by oxidative stress is connected various steps involved in many diseases. From the aspect, we investigated an antioxidant peptide to prevent inflammatory response against oxidant overexpression. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis, and the antioxidant properties of the hydrolysates were investigated using free radical scavenging activity by electron spin resonance (ESR) spectrometry. Papain hydrolysates, which showed clearly superior free radical scavenging activity, were further purified using consecutive chromatographic methods. Finally, a novel antioxidant peptide was obtained, and the sequence was identified as Ser-Leu-Pro-Ile-Gly-Leu-Met-Ile-Ala-Met at N-terminal. Oral administration of the peptide to mice effectively inhibited malondialdehyde (MDA) levels in a thiobarbituric acid reactive substances (TBARS) assay, and we also confirmed the antioxidative enzyme activities in superoxide dismutase (SOD) and glutathione-s-transferase (GST) assays. This is the first report of an antioxidant peptide derived from the hydrolysate of Mytilus coruscus, and also these results suggest that the peptide possesses potent antioxidant activity, and potential to enhance anti-inflammatory response. (C) 2013 Elsevier Ltd. All rights reserved.