Antioxidant and antimicrobial activities of Polygonum cognatum Meissn extracts
Antioxidant and antimicrobial activities of Polygonum cognatum Meissn extracts
复制标题
何首乌提取物的抗氧化和抗菌活性
DOI：
--
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
A. Kara
中科院分区：
文献类型：
--
作者：
A. Yildirim;A. Mavi;A. Kara
研究了野蓼乙醚、乙醇和热水提取物的体外抗氧化活性、还原力、清除DPPH自由基能力、总酚含量和抑菌活性。水提物的抗氧化活性最高。然而，在120小时孵育期间，亚油酸乳剂（0.02 M，pH 7.0）中含15 µg ml−1浸提液的样品之间无统计学显著差异（P > 0.05）。水提物的还原力最高，但其还原力明显低于抗坏血酸。最高的DPPH自由基清除活性在水提取物中发现，在100 µg ml−1干燥水提取物的浓度下，DPPH自由基清除率为50%，而在相同浓度的干燥乙醇提取物中，该值为12%。令人惊讶的是，在醚提取物中没有观察到DPPH自由基清除活性。在10 µg ml−1水、乙醇和乙醚提取物中，酚类化合物的浓度分别为0.48、0.50和0.01 µg ml−1没食子酸当量。乙醚和乙醇提取物对金黄色葡萄球菌和枯草芽孢杆菌有一定的抗菌活性。水浸提液对所研究的微生物未显示出抗菌活性。
© 2002化学工业协会
The antioxidant activities, reducing powers, 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical-scavenging activities, total phenolic compound contents and antimicrobial activities of ether, ethanol and hot water extracts of Polygonum cognatum Meissn were studied in vitro. The highest antioxidant activity was found in the water extract. However, there were no statistically significant differences among 15 µg ml−1 extract-containing samples in linoleic acid emulsion (0.02 M, pH 7.0) during 120 h of incubation (P > 0.05). The reducing power of the water extract was the highest, but its reducing power was markedly lower than that of ascorbic acid. The highest DPPH radical-scavenging activity was found in the water extract, with 50% DPPH radical scavenging at a concentration of 100 µg ml−1 dried water extract, while at the same concentration of dried ethanol extract the value was 12%. Surprisingly, no DPPH radical-scavenging activity was observed in the ether extract. The concentrations of phenolic compounds found were 0.48, 0.50 and 0.01 µg ml−1 gallic acid equivalent in 10 µg ml−1 water, ethanol and ether extracts respectively. The ether and ethanol extracts showed antimicrobial activity against Staphylococcus aureus and Bacillus subtilis. The water extract did not show antimicrobial activity against the studied micro-organisms.
© 2002 Society of Chemical Industry