Interpenetrating polymer network hydrogel scaffolds for artificial cornea periphery.
Interpenetrating polymer network hydrogel scaffolds for artificial cornea periphery.
复制标题
用于人工角膜周边的互穿聚合物网络水凝胶支架。
DOI：
10.1007/s10856-015-5442-2
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Frank,CurtisW
中科院分区：
文献类型：
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作者：
Parke-Houben,Rachel;Fox,CourtneyH;Zheng,LuoLuo;Waters,DaleJ;Cochran,JenniferR;Ta,ChristopherN;Frank,CurtisW
将聚乙二醇和聚丙烯酸的互穿聚合物网络（IPN）水凝胶序贯聚合制成了基于倒胶体晶体（ICCs）的三维支架。这种高强度，高含水量的IPN水凝胶可能适合用于人工角膜的应用。在人工角膜装置的外围形成一个高度多孔的、生物可整合的区域对于植入物的长期保留是至关重要的。ICC制造技术生产的支架具有良好的控制，可调的孔和通道尺寸。当细胞外基质蛋白表面功能化后，角膜成纤维细胞在IPN水凝胶支架上成功培养，证明了这些凝胶作为人工角膜多孔外周材料的可行性。使用变压扫描电子显微镜，无创地观察了有和没有细胞的多孔水凝胶在水合状态下的情况。
Three-dimensional scaffolds based on inverted colloidal crystals (ICCs) were fabricated from sequentially polymerized interpenetrating polymer network (IPN) hydrogels of poly(ethyleneglycol) and poly(acrylic acid). This high-strength, high-water-content IPN hydrogel may be suitable for use in an artificial cornea application. Development of a highly porous, biointegrable region at the periphery of the artificial cornea device is critical to long-term retention of the implant. The ICC fabrication technique produced scaffolds with well-controlled, tunable pore and channel dimensions. When surface functionalized with extracellular matrix proteins, corneal fibroblasts were successfully cultured on IPN hydrogel scaffolds, demonstrating the feasibility of these gels as materials for the artificial cornea porous periphery. Porous hydrogels with and without cells were visualized non-invasively in the hydrated state using variable-pressure scanning electron microscopy.