Physiological and morphological properties of neurons in sphincter of Oddi region of the guinea pig.
Physiological and morphological properties of neurons in sphincter of Oddi region of the guinea pig.
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DOI：
10.1152/ajpgi.1993.265.2.g258
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发表时间：
1993-08
期刊：
影响因子：
--
通讯作者：
D. G. Wells;G. Mawe
中科院分区：
文献类型：
--
作者：
D. G. Wells;G. Mawe
采用细胞内记录和染料注射的方法，对豚鼠Oddi括约肌(SO)区神经节内神经元进行了研究。根据生理特性，我们遇到了四种类型的神经元。最丰富的两种类型，强势和相态，具有相似的膜特征和形态，但可以通过它们的穗部特征来区分。紧张性细胞在整个去极化电流脉冲中出现尖峰，有时会自发活跃。无论刺激幅度或持续时间如何，时相细胞在电流脉冲开始时只激发一个动作电位。强直细胞和时相细胞均具有Dogiel I型形态。它们通常有一个很长的过程和几个从SOMA发出的很短的过程。NADPH黄递酶活性在形态相似的细胞中被显示，这表明一氧化氮可能是其中一些细胞的内在递质。后超极化延长的细胞(AH细胞)与肠道的1型/AH细胞相似的情况很少见。这一发现与该区域几乎没有Calbindin D免疫反应神经元的观察结果是一致的。被称为非尖峰神经元的第四类神经元不能产生动作电位，即使它们确实接受了突触输入。大多数强直相细胞至少接受一个烟碱快速兴奋性突触后电位(EPSP)。此外，还观察到慢的EPSP和抑制性突触后电位。大多数AH细胞只接受缓慢的兴奋性突触输入。
Intracellular recordings and dye injections were used to investigate neurons located in ganglia of the sphincter of Oddi (SO) region in guinea pigs. Four types of neurons were encountered based on physiological properties. The two most abundant types, tonic and phasic, had similar membrane characteristics and morphologies but yet could be differentiated by their spiking characteristics. Tonic cells spiked throughout a depolarizing current pulse and were sometimes spontaneously active. Phasic cells fired only a single action potential at the onset of a current pulse regardless of stimulus amplitude or duration. Both tonic and phasic cells had Dogiel type I morphologies. They typically had a single long process and several very short processes emanating from the soma. NADPH diaphorase activity was demonstrated in cells with similar morphologies, indicating that nitric oxide may be an intrinsic transmitter in some of these cells. Cells with a prolonged afterhyperpolarization (AH cells), similar to the type 1/AH cells of the gut, were rarely encountered. This finding was consistent with the observation that very few calbindin D-immunoreactive neurons exist in this region. Action potentials could not be generated in the fourth type of neuron, called nonspiking neurons, even though they did receive synaptic input. Most tonic and phasic cells received at least one nicotinic fast excitatory postsynaptic potential (EPSP). In addition, both slow EPSPs and inhibitory postsynaptic potentials were observed. Most AH cells received only slow excitatory synaptic input.