Surface plasmon resonance characterization of drug/liposome interactions
Surface plasmon resonance characterization of drug/liposome interactions
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DOI：
10.1016/s0003-2697(02)00278-6
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发表时间：
2002-11-01
影响因子：
2.9
通讯作者：
Myszka, DG
中科院分区：
文献类型：
--
作者：
Baird, CL;Courtenay, ES;Myszka, DG
使用Biacore的基于表面等离子体共振的生物传感器技术，我们开发了实验方案，并探测了研究药物与脂质体表面相互作用所需的测试条件。疏水烷烃表面上的脂质体捕获（Pioneer L1芯片）在pH、温度、脂质含量、胆固醇含量和缓冲液二甲亚砜浓度的可变条件下是可再现的和稳定的。重要的是，药物结合反应与捕获的脂质量成正比，而药物结合的动力学和反应的大小与药物的化学组成相关。在一般情况下，阴离子药物倾向于迅速从表面解离，而阳离子药物显示异质结合，这表明在脂质双层本身的分区。结果说明了如何表面等离子体共振可用于建立药物的被动转运特性。(C)2002 Elsevier Science（美国）。All rights reserved.
Using Biacore's surface plasmon resonance-based biosensor technology, we developed experimental protocols and probed test conditions required to study drugs interacting with liposome surfaces. Liposome capture on hydrophobic alkane surfaces (Pioneer L1 chip) was reproducible and stable under variable conditions of pH, temperature, lipid content, cholesterol content, and buffer dimethylsulfoxide concentration. Importantly, drug binding responses were directly proportional to the amount of lipid captured, while the kinetics of drug binding and the magnitude of the responses correlated with a drug's chemical composition. In general, anionic drugs tended to rapidly dissociate from the surface, while cationic drugs displayed heterogeneous binding, suggesting partitioning within the lipid bilayer itself. The results illustrate how surface plasmon resonance can be used to establish passive transport properties of drugs. (C) 2002 Elsevier Science (USA). All rights reserved.