REDUCTIVE DISSOLUTION OF FE(III) OXIDES BY PSEUDOMONAS SP 200
REDUCTIVE DISSOLUTION OF FE(III) OXIDES BY PSEUDOMONAS SP 200
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DOI：
10.1002/bit.260320902
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发表时间：
1988-10-20
影响因子：
3.8
通讯作者：
HOFFMANN, MR
中科院分区：
文献类型：
--
作者：
ARNOLD, RG;DICHRISTINA, TJ;HOFFMANN, MR
还原溶解的Fe（III）氧化物的动力学和机制进行了研究，在纯，分批培养假单胞菌。200。控制赤铁矿溶解动力学的主要因素是矿物表面积（或高能表面位点的浓度），配体浓度和细胞数。在氨三乙酸（NTA）的存在下，饱和动力学明显的还原溶解赤铁矿的关系。一个动力学表达式，其中整体铁还原率是功能相关的NTA和Fe（III）的浓度。添加NTA导致矿物（还原）溶解的微生物速率增加20倍。NTA作为一个桥接配体，穿梭呼吸电子从膜结合的微生物电子传递链的金属中心的氧化铁，或加速离开Fe（II）中心（结合到配体）从氧化物表面还原后的机制已被假定。实验结果表明，细胞-矿物接触是针铁矿还原溶解的必要条件。
The kinetics and mechanism of reductive dissolution of Fe(III) oxides were examined in pure, batch cultures of Pseudomonas sp. 200. Primary factors controlling hematite dissolution kinetics were mineral surface area (or concentration of high-energy surface sites), ligand concentration, and cell number. In the presence of nitrilotriacetic acid (NTA), saturation kinetics were apparent in the relationship governing reductive dissolution of hematite. A kinetic expression was developed in which overall iron-reduction rate is functionally related to the concentrations of both NTA and Fe(III). Addition of NTA resulted in a 20-fold increase in the microbial rate of mineral (reductive) dissolution. Mechanisms in which NTA served as a bridging ligand, shuttling respiratory electrons from the membrane-bound microbial electron transport chain to the metal center of the iron oxide, or accelerated the departure of Fe(II) centers (bound to ligand) from the oxide surface following reduction have been postulated. Experimental results indicated that cell-mineral contact was essential for reductive dissolution of goethite.