Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
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DOI：
10.4049/jimmunol.150.2.655
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发表时间：
1993-01
影响因子：
4.4
通讯作者：
A. Scheynius;R. Camp;E. Puré
中科院分区：
文献类型：
--
作者：
A. Scheynius;R. Camp;E. Puré
我们研究了白细胞功能相关分子-1（CD 11 a/CD 18）和细胞间粘附分子-1（CD 54）单克隆抗体对2，4-二硝基-1-氟苯致敏的CD 2F 1小鼠迟发型超敏反应的影响。与对照动物相比，在耳激发时腹膜内注射抗CD 11 a的mAb FD 441.8几乎完全抑制了耳肿胀。攻毒前给予抗CD 54 mAb、3E 2或YN 1/1.7.4可使迟发型超敏反应降低约50%。耳肿胀的减少反映了抗CD 11 a处理动物耳中水肿和细胞浸润的显著抑制，以及抗CD 54处理的部分抑制。此外，在注射正常IgG的致敏小鼠中，攻毒后24 h从引流淋巴结中回收的细胞数量增加3倍，但在抗CD 11 a处理的小鼠中减少，在抗CD 54处理的动物中部分减少。免疫组织化学和流式细胞术分析表明，在体内给药的抗CD 11 a单克隆抗体与注射和挑战后24小时的淋巴结中的大多数细胞的表面，而抗细胞间粘附分子-1单克隆抗体反应优先与血管内皮。结论：白细胞功能相关分子-1和细胞间粘附分子-1有助于产生最佳迟发型超敏反应。
We have investigated the effect of the administration of mAb against leukocyte function-associated molecule-1 (CD11a/CD18) and intercellular adhesion molecule-1 (CD54) on the delayed-type hypersensitivity reaction in 2,4-dinitro-1-fluorobenzene-sensitized CD2F1 mice. An i.p. injection of the mAb FD441.8 against CD11a at the time of ear challenge led to an almost complete inhibition of ear swelling compared with control animals. Administration of anti-CD54 mAb, 3E2 or YN1/1.7.4, before challenge, resulted in approximately 50% reduction of the delayed-type hypersensitivity response. The decrease in ear swelling reflected a profound inhibition of the edema and the cell infiltration in ears from animals treated with anti-CD11a, and a partial inhibition with anti-CD54 treatment. In addition, the threefold increase in the number of cells recovered from the draining lymph nodes 24 h after challenge in sensitized mice injected with normal IgG was ablated in mice treated with anti-CD11a and partially reduced in anti-CD54-treated animals. Immunohistochemistry and flow cytometry analysis demonstrated that the in vivo administered anti-CD11a mAb was associated with the surface of the majority of the cells in the lymph nodes 24 h after injection and challenge, whereas the anti-intercellular adhesion molecule-1 mAb reacted preferentially with the vascular endothelium. It is concluded that leukocyte function-associated molecule-1 and intercellular adhesion molecule-1 contribute to the generation of an optimal delayed-type hypersensitivity response.