Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism
Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism
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DOI：
10.1016/j.envpol.2016.11.010
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发表时间：
2017-01-01
影响因子：
8.9
通讯作者：
Tauler, Roma
中科院分区：
文献类型：
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作者：
Gomez-Canela, Cristian;Prats, Eva;Tauler, Roma
在这项工作中，通过液相色谱与高分辨率质谱联用评估了毒死蜱暴露对斑马鱼肌肉代谢特征的影响。提出了基于感兴趣区域选择和多元曲线分辨率交替最小二乘的不同化学计量工具，用于分析不同暴露实验中生成的复杂数据集。方差分析 代谢物峰轮廓区域变化的同时成分分析显示毒死蜱浓度和暴露时间依赖性变化，清楚地区分暴露和非暴露样品以及短暴露时间（2 小时）和长暴露时间（6 小时或 24 小时）。观察到的 50 种肌肉代谢物浓度的变化表明氧化应激的诱导、神经递质代谢的全面破坏以及肌肉的衰竭。这三种作用与毒死蜱的毒性密切相关。 (C) 2016 Elsevier Ltd. 保留所有权利。
In this work the effect of chlorpyrifos exposure on metabolic profiles of zebrafish muscle was evaluated by liquid chromatography coupled to high resolution mass spectrometry. Different chemometric tools based on the selection of Regions of Interest and on Multivariate Curve-Resolution-Alternating Least Squares are proposed for the analysis of the complex data sets generated in the different exposure experiments. Analysis of Variance Simultaneous Component Analysis of changes on metabolite peak profile areas showed significant chlorpyrifos concentration and exposure time-dependent changes, clearly differentiating between exposed and non-exposed samples and between short (2 h) and long exposure times (6 h or 24 h). The changes observed in the concentrations of 50 muscle metabolites are indicative of induction of oxidative stress, of a general disruption of neurotransmitter metabolism, and of muscle exhaustion. These three effects are intimately related to the toxicity of chlorpyrifos. (C) 2016 Elsevier Ltd. All rights reserved.