Genetic Transformation as a tool for detection of Neisseria gonorrhoeae
Genetic Transformation as a tool for detection of Neisseria gonorrhoeae
复制标题
基因转化作为检测淋病奈瑟菌的工具
DOI：
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发表时间：
1976
影响因子：
9.4
通讯作者：
G. Heym
中科院分区：
文献类型：
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作者：
A. Janik;E. Juni;G. Heym
一种快速检测淋病奈瑟菌的方法，该方法利用来自该生物临床分离菌株的脱氧核糖核酸样品转化特定淋病奈瑟菌菌株的营养突变体的能力。淋病，已被描述。除了使用分离培养物外，转化脱氧核糖核酸还可以直接从粘附在宫颈或尿道拭子上的物质中获得。将拭子转移到诊断实验室的时间间隔不是一个重要因素。不必在原代分离平板上使用纯培养物来获得确定的结果。当在一组标准化条件下使用一种突变菌株时，非淋病奈瑟菌以及大量常见的不相关细菌不会对转化试验产生反应或干扰。整个检测可在24小时内完成。还表明，N.在本研究中使用的淋病奈瑟氏菌是有能力进行遗传转化的，尽管T4型细胞的转化频率明显低于相同菌株的T2型细胞。
A rapid method for the detection of Neisseria gonorrhoeae, making use of the ability of deoxyribonucleic acid samples from clinically isolated strains of this organism to transform nutritional mutants of a particular strain of N. gonorrhoeae, has been described. In addition to using isolated cultures, transforming deoxyribonucleic acid can be obtained directly from the material that adheres to swabs of the cervix or the urethra. The time interval for transfer of swabs to the diagnostic laboratory is not a significant factor. It is not necessary to use pure cultures on primary isolation plates to obtain definitive results. Nongonorrhoeae neisserias, as well as a large variety of commonly encountered unrelated bacteria, do not react or interfere in the transformation assay when using one of the mutant strains under a standardized set of conditions. The entire assay can be completed in less than 24 h. It has also been shown that type T4 cells of the strain of N. gonorrhoeae employed in the present study are competent for genetic transformation, although type T4 cells are transformed at a significantly lower frequency than are type T2 cells of the same strain.