THE INDUCTION OF SPECIFIC DYNAMIC ACTION IN CHANNEL CATFISH BY INFUSION OF ESSENTIAL AMINO-ACIDS
THE INDUCTION OF SPECIFIC DYNAMIC ACTION IN CHANNEL CATFISH BY INFUSION OF ESSENTIAL AMINO-ACIDS
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DOI：
10.1086/physzool.64.1.30158524
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
CAMERON, JN
中科院分区：
文献类型：
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作者：
BROWN, CR;CAMERON, JN
比动态作用 (SDA) 定义为进食后氧气消耗量的增加。 这种现象已经被观察了很多年，但仍然没有得到很好的理解。 然而，有人认为与生长相关的代谢消耗是 SDA 的主要致病因素。 本研究的目的是确定是否可以通过将氨基酸混合物直接注入斑点叉尾鮰的血液中来人工诱导 SDA。 输注相当于标准日粮蛋白质（40 g kg-1）的必需氨基酸（EAA）混合物会导致氧气消耗和氨排泄显着增加。 血浆氨基酸浓度随着氧气消耗和氨排泄率而增加。 D-必需氨基酸混合物的输注不会导致氧气消耗或氨排泄显着增加。 因此，输注 EAA 后新陈代谢的增加不是由于渗透压或生理应激所致。 在输注 EAA 之前给予蛋白质合成抑制剂放线菌酮可抑制 SDA 特征性的 O2 消耗率增加。 这进一步证明了 SDA 与蛋白质合成之间的关系。
Specific dynamic action (SDA) is defined as the increase in O2 consumption following the ingestion of a meal. This phenomenon has been observed for many years but is still not well understood. It has been suggested, however, that the metabolic cost associated with growth is the prime causative agent of SDA. The purpose of this study was to determine whether SDA could be artificially induced in the channel catfish by infusing an amino acid mixture directly into the blood-stream. The infusion of an essential amino acid (EAA) mixture equivalent to that in the protein of a standard ration (40 g kg-1) resulted in significant increases in O2 consumption and ammonia excretion. Plasma amino acid concentrations increased in accordance with O2 consumption and ammonia excretion rates. The infusion of a D-essential amino acid mixture did not cause a significant increase in O2 consumption or ammonia excretion. The increase in metabolism following EAA infusion was therefore not due to osmotic or physiological stress. The administration of the protein synthesis inhibitor cycloheximide before the infusion of EAAs inhibited the increased O2 consumption rate characteristic of SDA. This offers further evidence of the relationship between SDA and protein synthesis.