DNA Replication Control During Drosophila Development: Insights into the Onset of S Phase, Replication Initiation, and Fork Progression
DNA Replication Control During Drosophila Development: Insights into the Onset of S Phase, Replication Initiation, and Fork Progression
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DOI：
10.1534/genetics.115.186627
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发表时间：
2017-09-01
期刊：
影响因子：
3.3
通讯作者：
Orr-Weaver, Terry L.
中科院分区：
文献类型：
--
作者：
Hua, Brian L.;Orr-Weaver, Terry L.
DNA复制的适当控制对于确保细胞增殖期间的基因组完整性至关重要。此外，发育过程中DNA复制程序的差异调节可以改变基因拷贝数，从而影响细胞大小和基因表达。黑腹果蝇是一种功能强大的生物体，可以在各种分化的细胞和组织类型中研究不同细胞周期背景下DNA复制的发育控制。此外，果蝇还提供了几种发育调控的复制模型，以剖析基因组中基于复制的拷贝数变化的分子机制，包括差异复制不足和基因扩增。在这里，我们回顾了关键的发现和我们目前的理解的原型复制程序的背景下，以及复制不足和差异基因扩增的DNA复制的发展控制。我们专注于使用这后两个复制系统来描绘许多的分子机制，这些机制是复制起始和分叉延伸的发育控制的基础。
Proper control of DNA replication is critical to ensure genomic integrity during cell proliferation. In addition, differential regulation of the DNA replication program during development can change gene copy number to influence cell size and gene expression. Drosophila melanogaster serves as a powerful organism to study the developmental control of DNA replication in various cell cycle contexts in a variety of differentiated cell and tissue types. Additionally, Drosophila has provided several developmentally regulated replication models to dissect the molecular mechanisms that underlie replication-based copy number changes in the genome, which include differential underreplication and gene amplification. Here, we review key findings and our current understanding of the developmental control of DNA replication in the contexts of the archetypal replication program as well as of underreplication and differential gene amplification. We focus on the use of these latter two replication systems to delineate many of the molecular mechanisms that underlie the developmental control of replication initiation and fork elongation.