Impact of unhygienic conditions during slaughtering and processing on spread of antibiotic resistant Escherichia coli from poultry
Impact of unhygienic conditions during slaughtering and processing on spread of antibiotic resistant Escherichia coli from poultry
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DOI：
10.4081/mr.2017.7330
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发表时间：
2017-01-01
影响因子：
1.5
通讯作者：
Kashif, Muhammad
中科院分区：
文献类型：
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作者：
Amir, Mamoona;Riaz, Muhammad;Kashif, Muhammad
大肠杆菌的抗生素耐药性是一个全球性的健康问题。研究了耐药大肠杆菌在肉仔鸡中交叉污染的各种可能途径。在家禽店从肉鸡粪便中提取大肠杆菌。从当地家禽加工市场收集各种样品类别，即家禽粪便、肉类（每种n=225）、屠宰手、消费者手、屠宰刀、罐、自来水、屠体、饲料和饮用水（每种n=50）。对样本进行E.大肠埃希菌、对10种抗生素的耐药性及四环素耐药基因的存在。粪便样本的菌落数（4.1 × 10（4）CFU/g）最高，而肉类样本的菌落数（1.9 × 10（4）CFU/g）最低。消费者手（6%）和自来水（12%）样本中的流行率较低。大肠杆菌阳性率为92%，肉鸡饮水阳性率为86%。8个样品类别的分离株对土霉素的耐药率较高（>= 90%）。平均而言，约94%的菌株从不同的样本类别具有多重耐药（MDR）。四环素耐药基因（tetA和tetB）被确定在所有的样本类别，除了消费者的手和自来水分离。四环素耐药基因在粪便分离株（42%）中的分布显著高于肉类分离株（25%）。该研究描述了耐药大肠杆菌的传播。大肠杆菌在肉鸡肉通过所有研究途径的屠宰周边污染。通过严格监控家禽养殖场抗生素的使用，采用卫生屠宰防止交叉污染，以及对市场上的肉类进行严格的耐药大肠杆菌筛查，可以缓解这一问题。杆菌
Antibiotic resistance in Escherichia coli is a global health concern. We studied all possible routes of cross contamination of broiler meat with resistant E. coli from broiler feces at poultry shops. Various sample categories namely poultry feces, meat (n=225 for each), slaughterer hands, consumer hands, slaughterer knife, canister, tap water, carcass, feed and drinking water (n=50 for each) were collected from local poultry processing market. Samples were screened for prevalence of E. coli, resistance of isolates against ten antibiotics and presence of tetracycline-resistance genes in the isolates. Fecal samples had greatest colony count (4.1x10(4) CFU/g) as compared to meat (1.9x10(4) CFU/g) samples. Samples of consumer hands (6%) and tap water (12%) had less prevalence percentages of E. coli as compared to slaughterer hands (92%) and drinking water of broiler (86%). Isolates of eight sample categories had high resistant rate (>= 90%) against oxytetracycline. On average, about 94% of the isolates from various sample categories possessed multidrug-resistance (MDR). Tetracycline-resistance genes (tetA and tetB) were identified in all sample categories except isolates of consumer hands and tap water. The distribution of tetracycline-resistance genes was significantly greater in fecal isolates (42%) than meat isolates (25%). The study depicted the spread of resistant E. coli in broiler meat through all studied routes of contamination of slaughtering periphery. This problem can be mitigated by strict monitoring of antibiotics use at poultry farms, prevention of cross contamination by adopting hygienic slaughter and vigorously screening the market meat for resistant E. coli.