cAMP acts in synergy with E1A protein to activate transcription of the adenovirus early genes E4 and E1A.
cAMP acts in synergy with E1A protein to activate transcription of the adenovirus early genes E4 and E1A.
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cAMP 与 E1A 蛋白协同作用，激活腺病毒早期基因 E4 和 E1A 的转录。
DOI：
10.1101/gad.2.12a.1517
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发表时间：
1988
影响因子：
10.5
通讯作者：
Shenk,T
中科院分区：
文献类型：
--
作者：
Engel,DA;Hardy,S;Shenk,T
几个E1A诱导的腺病毒早期基因的转录控制区含有类似于几个细胞cAMP诱导基因的cAMP反应元件的序列。用野生型腺病毒感染cAMP应答细胞系S49，发现在用二丁酰cAMP处理后，含有升高水平的由所有测试的早期基因（E4、E1A和E1B）编码的mRNA。这种效应是在转录激活水平上。cAMP对E4和E1A转录的影响在感染野生型病毒的细胞中比在感染缺乏功能性E1A蛋白的病毒的细胞中更大。cAMP与E1A组合产生的诱导作用大于每种诱导剂单独所实现的增加的产物。因此，cAMP与E1A协同作用以诱导E4和E1A基因的最大转录。这些数据表明，E1A或E1A刺激的事件可以在功能上与细胞中的cAMP信号转导的靶点相互作用，以诱导腺病毒早期基因的转录。
The transcriptional control regions of several E1A-inducible adenovirus early genes contain sequences similar to the cAMP response element of several cellular cAMP-inducible genes. The cAMP-responsive cell line S49 was infected with wild-type adenovirus and found to contain elevated levels of mRNAs encoded by all early genes tested (E4, E1A, and E1B), following treatment with dibutyryl cAMP. This effect was at the level of transcriptional activation. The effect of cAMP on E4 and E1A transcription was greater in cells infected with wild-type virus than in cells infected with virus that lacked functional E1A proteins. cAMP in combination with E1A generated a greater induction than the product of the increases achieved by each inducer alone. Therefore, cAMP acted in synergy with E1A to induce maximally transcription of the E4 and E1A genes. These data suggest that E1A or E1A-stimulated events can interact functionally with targets of cAMP signaling in the cell to induce transcription of the adenovirus early genes.