Impairment of male reproductive function after sleep deprivation
Impairment of male reproductive function after sleep deprivation
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DOI：
10.1016/j.fertnstert.2015.02.002
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发表时间：
2015-05-01
影响因子：
6.7
通讯作者：
Andersen, Monica L.
中科院分区：
文献类型：
--
作者：
Alvarenga, Tathiana A.;Hirotsu, Camila;Andersen, Monica L.
目的：探讨睡眠不足对雄性大鼠性行为、激素水平、精子参数和睾丸特异性基因表达的影响。设计：实验研究。环境：动物实验室。动物：雄性成年威斯塔-汉诺威大鼠。干预：有过性经验的大鼠进行矛盾睡眠剥夺（PSD） 96小时或睡眠限制（SR） 21天或在其家中笼子中作为对照组（CTRL）。主要观察指标：评价性行为、激素水平、精子参数、应激和一氧化氮相关基因的表达。结果：与对照组相比，PSD显著降低了性行为，而SR没有影响。PSD组的睾酮水平明显低于CTRL组。PSD组和SR组精子存活率均低于CTRL组。与CTRL组相比，PSD组的活精子数下降幅度大于SR组。在睾丸基因表达方面，与对照组相比，PSD和SR均导致iNOS和羟基类固醇11 β -脱氢酶1表达增加。这些变化在PSD组中更为明显。与对照组相比，PSD组内皮型一氧化氮合酶表达明显增加。二甲基精氨酸二甲氨基水解酶1和酪蛋白激酶2 β多肽的表达未见变化。结论：睡眠不足可以促进大鼠雄性生殖系统的显著变化，特别是通过干扰睾丸一氧化氮通路部分影响精子功能。(C) 2015年美国生殖医学学会。
Objective: To evaluate the influence of sleep loss on sexual behavior, hormone levels, sperm parameters, and testis-specific gene expression in male rats.Design: Experimental research.Setting: Animal laboratory.Animal(s): Male adult Wistar-Hannover rats.Intervention(s): Sexually experienced rats were subjected to paradoxic sleep deprivation (PSD) for 96 hours or sleep restriction (SR) for 21 days or kept in their home cage as control (CTRL).Main Outcome Measure(s): Sexual behavior, hormone levels, sperm parameters and expression of stress and nitric oxide-related genes were evaluated.Result(s): PSD significantly decreased sexual behavior compared with the CTRL group, whereas SR had no effect. The PSD group had significantly lower testosterone levels than the CTRL group. Both PSD and SR groups had lower sperm viabilities than the CTRL group. The decrease in the number of live sperm compared with the CTRL group was larger in the PSD group than in the SR group. Regarding testicular gene expression, both PSD and SR led to an increase of iNOS and hydroxysteroid 11 beta-dehydrogenase 1 expressions compared with the CTRL group. These changes were more pronounced in the PSD group. A significant increase in endothelial nitric oxide synthase expression was observed in the PSD groups compared with the CTRL group. No changes were observed in dimethylarginine dimethylaminohydrolase 1 and casein kinase 2 beta-polypeptide expressions.Conclusion(s): Sleep loss can promote marked changes in the male reproductive system of rats, particularly affecting spermatic function in part by interfering in the testicular nitric oxide pathway. (C) 2015 by American Society for Reproductive Medicine.