Effects of liver‐regulating herb compounds on testicular morphological and ultrastructural changes in varicocele rats through SCF/C‐KIT pathway
Effects of liver‐regulating herb compounds on testicular morphological and ultrastructural changes in varicocele rats through SCF/C‐KIT pathway
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DOI：
10.1111/and.13658
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发表时间：
2020-06
期刊：
影响因子：
2.4
通讯作者：
Xianfeng Lu;Jianrong Liu;Haizhen Yin;C. Ding;Yimin Wang;Fang Zhang;Guorong Jin;Yuehong Ma;Lina Dong;Qin Qin-Qin
中科院分区：
文献类型：
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作者：
Xianfeng Lu;Jianrong Liu;Haizhen Yin;C. Ding;Yimin Wang;Fang Zhang;Guorong Jin;Yuehong Ma;Lina Dong;Qin Qin-Qin
调肝中药复方对精索静脉曲张（VC）患者的精子质量及男性生育力有较好的改善作用。但LRHC对VC的作用机制尚不清楚。本研究探讨了LRHC对VC大鼠睾丸组织形态学和超微结构变化及干细胞因子（SCF）和C-KIT表达的影响。将24只雄性大鼠分为3组，每组8只大鼠，分别为假手术组、精索静脉曲张组和LRHC组。取睾丸标本分别行光镜和透射电镜检查。Western blot检测SCF/C-KIT的表达。结果表明，VC大鼠曲细精管受损，细胞数量减少。超微结构改变，如固有层厚度增加，支持细胞，精母细胞和精子细胞空泡化，精子头部和线粒体异常。而LRHC处理组大鼠的曲细精管结构与假手术组大鼠的曲细精管结构相同，组织致密，支持细胞、间质细胞和生殖细胞的超微结构发育良好。LRHC可改善VC的组织形态和超微结构。此外，在VC大鼠睾丸组织中观察到SCF和C-KIT的异常表达，LRHC使其恢复正常水平。
Liver‐regulating herb compound (LRHC) has good effects on improving sperm quality and male fertility of varicocele (VC) patients. But the mechanism of LRHC on VC is still not clear. This study explored the effects of LRHC on histomorphological and ultrastructural changes and expression of stem cell factor (SCF) and C‐KIT of VC rat testis. Twenty‐four male rats were divided into three groups with eight rats in each group as sham, varicocele and LRHC groups. Testis specimens were collected for light microscopy and transmission electron microscopy respectively. The expression of SCF/C‐KIT was detected with Western blot. Results showed that seminiferous tubules in VC rats were damaged and cell numbers were decreased. Ultrastructural alterations were observed, such as increased thickness of lamina propria, vacuolation in Sertoli cells, spermatocytes and spermatids, and abnormal head and mitochondria in spermatozoa. While in LRHC‐treated rats, the architectures of seminiferous tubules were as organised and compact as that of sham animals, and ultrastructure of Sertoli, Leydig and germ cells developed well. LRHC ameliorated histological appearance and ultrastructure by VC. In addition, the abnormal expression of SCF and C‐KIT were observed in testicular tissues from rats with VC, which were brought back to normal level by LRHC.