Drug Delivery of Cisplatin to Breast Cancer by Polybutylcyanoacrylate Nanoparticles
Drug Delivery of Cisplatin to Breast Cancer by Polybutylcyanoacrylate Nanoparticles
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DOI：
10.1002/adv.21709
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发表时间：
2018-04-01
影响因子：
3.1
通讯作者：
Shahmabadi, Hasan Ebrahimi
中科院分区：
文献类型：
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作者：
Esfahani, Maedeh Koohi Moftakhari;Alavi, Seyed Ebrahim;Shahmabadi, Hasan Ebrahimi
乳腺癌是最常见的癌症，也是全球女性癌症相关死亡的第二大原因。疾病的化疗因随后的复发和转移而受阻。纳米粒子作为药物载体已显示出良好的应用前景。成功构建了顺铂聚氰基丙烯酸丁酯纳米粒(NPs)，并在原位乳腺癌模型上对其疗效和毒性进行了评价。采用细乳液聚合技术制备了顺铂PBCA纳米粒。用光子相关光谱、电感耦合等离子体发射光谱(ICPOES)和分光光度技术对NPs进行了表征。采用四甲基偶氮唑盐比色法检测纳米微球的细胞毒性。为了评价顺铂负载的PBCA纳米粒的疗效，采用了一种原位乳腺肿瘤模型。与标准药物相比，纳米微球的细胞活力在最初的24小时内显著下降了75%。体内研究表明，顺铂处理的小鼠与PBCA纳米粒结合的数量明显多于标准药物接受者(8对5只)。考虑到体重减轻是毒性效应，纳米地毯接受者的体重减轻也明显低于药物接受者组(20%对26%)。
Breast cancer is the most common cancer and the second cause of cancer-related death in women worldwide. Chemotherapy of disease is impeded by subsequent relapse and metastasis. Nanoparticles as drug carriers have been shown the promising results. Cisplatin-loaded polybutylcyanoacrylate (PBCA) nanoparticles (NPs) were successfully constructed, and its efficacy and toxicity were evaluated on an orthotopic breast cancer model. Cisplatin-loaded PBCA NPs were prepared by miniemulsion polymerization technique. NPs were characterized with photon correlation spectroscopy, inductively coupled plasma optical emission spectrometry (ICP-OES) and spectrophotometry techniques. MTT assay was used to evaluate the cytotoxicity of nanodrug. To evaluate the efficacy of Cisplatin-loaded PBCA NPs an orthotopic model of breast tumor was employed. The cell viability of nanodrug compared to that of the standard drug had a significant decrease by 75% at the first 24h. In vivo studies showed that the number of mice treated with Cisplatin bound to PBCA NPs was significantly more than the standard drug receivers (8 against 5 mice). Considering the body weight loss as toxicity effects, nanodrug receivers were also shown significantly lesser body weight loss compared to drug receivers groups (20 against 26%).